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Home Antibody All anti-ACAT2 antibodies

Anti-ACAT2 TRUEMAB Antibody clone 7B1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501221
  • ACAT2 mouse monoclonal antibody, clone 7B1
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417006) , 20ug Explanation
100ul 325 In Stock
CF501221
  • Carrier-free (BSA/glycerol-free) ACAT2 mouse monoclonal antibody, clone 7B1
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(6)
IF(1)
FC(1)
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Also for ACAT2 (NM_005891)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ACAT2 (NP_0058826) produced in HEK293T cell.
Clone Nameclone 7B1 IsotypeIgG1
Species ReactivityHuman , Dog , Rat Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
Predicted MW Explanation 41.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens acetyl-CoA acetyltransferase 2 (ACAT2)
Alternative NameOTTHUMP00000017527; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 2
Database LinkNP_005882
Entrez Gene 39 Human
Entrez Gene 308100 Rat
Entrez Gene 484063 Dog
FunctionThe product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. [provided by RefSeq].
Related PathwayDruggable Genome Fatty acid metabolismSynthesis and degradation of ketone bodiesValine, leucine and isoleucine degradationLysine degradationTryptophan metabolismPyruvate metabolismMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501221)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501221)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501221)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501221)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501221)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501221)
IF Image
Anti-ACAT2 mouse monoclonal antibody (TA501221) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2(RC201821).
FC Image
Flow cytometric Analysis of Hela cells, using anti-ACAT2 antibody(TA501221),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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