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Anti-ACAT2 TRUEMAB Antibody clone 3A11

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA501217
  • anti-ACAT2 mouse monoclonal antibody, clone 3A11
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417006) , 20ug Explanation
100ul $325 In Stock
CF501217
  • Carrier-free (BSA/glycerol-free) ACAT2 mouse monoclonal antibody, clone 3A11
100ug $450 3-4 weeks
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WB(2)
IHC(3)
IF(2)
FC(1)
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Also for ACAT2 (NM_005891)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human ACAT2(NP_005882) produced in HEK293T cell.
Clone Nameclone 3A11 IsotypeIgG2b
Species ReactivityHuman Concentration0.88 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
Predicted MW Explanation 41.2 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens acetyl-CoA acetyltransferase 2 (ACAT2), transcript variant 1
Alternative NameOTTHUMP00000017527; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-Coenzyme A acetyltransferase 2
Database LinkNP_005882
Entrez Gene 39 Human
FunctionThe product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
Related PathwayDruggable Genome Fatty acid metabolismSynthesis and degradation of ketone bodiesValine, leucine and isoleucine degradationLysine degradationTryptophan metabolismPyruvate metabolismMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACAT2 (RC201821, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACAT2 .
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ACAT2 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501217, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501217, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-ACAT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501217, Dilution 1:50)
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Anti-ACAT2 mouse monoclonal antibody (TA501217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ACAT2 (RC201821 ).
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Immunofluorescent staining of HT29 cells using anti-ACAT2 mouse monoclonal antibody (TA501217).
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HEK293T cells transfected with either RC201821 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ACAT2 antibody(TA501217), and then analyzed by flow cytometry.
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