Aggrecan (ACAN) Rabbit Polyclonal Antibody

CAT#: TA336492

Rabbit Polyclonal Aggrecan Neoepitope Antibody

Product Images

Immunocytochemistry/Immunofluorescence: Aggrecan Neoepitope Antibody TA336492 - Detection of ACAN in OA cartilage using TA336492.

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  Immunohistochemistry: Aggrecan Neoepitope Antibody TA336492 - Analysis of ACAN in mouse cartilage with hematoxylin counterstain.

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  Western Blot: Aggrecan Neoepitope Antibody TA336492 - Analysis of ACAN in U2OS cell lysate.

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  Western Blot: Aggrecan Neoepitope Antibody TA336492 - Analysis of ACAN in L929 whole cell extract using TA336492.

Specifications

Product Data

• Applications: FC, ICC/IF, IHC, WB
• Recommended Dilution: Immunohistochemistry-Frozen, Flow Cytometry, Immunohistochemistry-Paraffin: 1:1000, Immunohistochemistry: 1:1000, Immunocytochemistry/ Immunofluorescence: 1:20-1:100, Western Blot: 1:1000
• Reactivities: Human, Mouse, Rat, Bovine, Canine, Porcine
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a region of human Aggrecan (within residues 350-400). [UniProt# P16112]
• Formulation: PBS, 30% glycerol, 0.1% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 61 kDa
• Gene Name: aggrecan
• Database Link:
  NP_001126
  Entrez Gene 11595 MouseEntrez Gene 58968 RatEntrez Gene 176 Human
  UniProt ID P16112
• Background: Aggrecan Neoepitope (ACAN) is a proteoglycan belonging to aggrecan/versican proteoglycan family and is a major component of extracellular matrix of cartilagenous tissues. ACAN proteoglycan is mainly consists of chondroitin sulfate, however, it also contains keratan sulfate chains, N/O-linked oligosaccharides. Aggrecanase mediated cleavage results in ACAN fragments release from articular cartilage into synovial fluid during different stages of human osteoarthritis. ACAN is a secreted protein found in extracellular space/extracellular matrix and is more or less restricted to cartilages where it interact with FBLN1 as well as COM for serving the role of resisting compression in cartilage. ACAN binds to hyaluronic acid via an N-terminal globular region with high avidity and its expression occurs in chondrocytes throughout the developing skeleton. ACAN defects have been linked to spondyloepiphyseal dysplasia type Kimberley (SEDK), spondyloepimetaphyseal dysplasia aggrecan type (SEMD-ACAN), osteochondritis dissecans short stature and early-onset osteoarthritis (OD).
• Synonyms: AGC1; AGCAN; CSPG1; CSPGCP; MSK16; SEDK
• Note: This Aggrecan Neoepitope antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry-paraffin embedded sections, and Western Blot. In WB a band is detected at ~61 kDa representing the neoepitope sequence (NITEGE) generated by aggrecanase-mediated cleavage at Glu373-Ala374 of aggrecan core proteins. Use in Immunohistochemistry-Frozen and Flow Cytometry reported in scientific literature (PMID 21482542)