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Anti-ACADM Antibody EPR3708
Also for ACADM (NM_000016)
|A synthetic peptide corresponding to residues in human ACADM was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC: 1:100 - 250, IHC: 1:100 - 250, IP: 1:10 - 100, WB: 1:10,000-50,000,
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Homo sapiens acyl-CoA dehydrogenase, C-4 to C-12 straight chain (ACADM), transcript variant 1|
|ACAD1; MCAD; MCADH|
|Medium-chain specific acyl-CoA dehydrogenase (ACADM) is a homotetramer enzyme of the mitochondrial flavoenzyme family that catalyzes the initial reaction in medium chain fatty acid beta-oxidation (1, 2). ACADM is specific for acyl chain lengths of 4 to 16 (3). It utilizes the electron transfer flavoprotein (ETF) as an electron acceptor that transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (3). Defects in the ACADM gene cause medium-chain acyl-CoA dehydrogenase deficiency (MCADD), a metabolic disorder characterized by fasting hypoglycemia (3). Mutation analysis can diagnose MCADD, which is currently screened in newborns, since its monogene may be modified by genetic variations (4).|
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Western blot - ACADM antibody [EPR3708]; All lanes : Anti-ACADM antibody [EPR3708] at 1/10000 dilution.Lane 1 : Human heart lysate.Lane 2 : fetal liver lysate.Lane 3 : HeLa cell lysate.Lane 4 : HepG2 cell lysate.Lane 5 : K562 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-Rabbit Ig at 1/2000 dilution.Predicted band size : 47 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - ACADM antibody [EPR3708]; ab92461, at 1/100 dilution, staining ACADM in formalin-fixed, paraffin-embedded Human liver tissue by immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - ACADM antibody [EPR3708]; Immunofluorescent staining of ACADM in HeLa cells using ab92461 at 1/100 dilution.