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Anti-ABL2 GAT Antibody Polyclonal
Generated by Genomic Immunization
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Also for ABL2 (NM_007314)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|WB, ELISA, IHC, IF
|| WB 1:5000~20000,ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens ABL proto-oncogene 2, non-receptor tyrosine kinase (ABL2), transcript variant b|
Entrez Gene 27 Human
|This gene encodes a member of the Abelson family of nonreceptor tyrosine protein kinase. The protein is highly similar to the ABL1 protein, including the tyrosine kinase, SH2 and SH3 domains, and has a role in cytoskeletal rearrangements by its C-terminal F-actin- and microtubule-binding sequences. This gene is expressed in both normal and tumor cells, and is involved in translocation with the ETV6 gene in leukemia. Multiple alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq]|
|Protein KinaseDruggable Genome ErbB signaling pathwayViral myocarditis|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABL2 (RC213232, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ABL2.
Western Blot: ABL2 Antibody - Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Ln 2, RT-4 Ln 3, U-251MG sp Ln 4, Human Plasma Ln 5, Liver Ln 6, Tonsil, Target weight: 128, 116, 110 (splice variants) Validation score: 2.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells. Antibody dilution: 1:350. Image and statement courtesy of the Human Protein Atlas (HPA).
Immunofluorescent staining of human cell line U-251 MG shows positivity in nucleus but excluded from the nucleoli. Antibody dilution: 1:87. Image and statement courtesy of the Human Protein Atlas (HPA).