Home Antibody All anti-AAK1 antibodies
Also for AAK1 (NM_014911)
|Aak1 antibody was raised against a 20 amino acid peptide near the amino terminus of the human Aak1.|
|Human, Mouse, Rat
||ELISA, WB: 1 - 2 ug/ml, ICC: 5 ug/ml, IF: 20 ug/ml
|PBS containing 0.02% sodium azide.|
|Affinity chromatography purified via peptide column
|Homo sapiens AP2 associated kinase 1 (AAK1)|
|AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.|
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Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 ug/mL.