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OriGene Antibodies in recent publications
Comparison of the dose–response relationship of radiation-induced apoptosis in the hippocampal dentate gyrus and intestinal crypt of adult mice Radiat Prot Dosimetry, Mar 2012; 148: 492 - 497. [LGR5 ]

Tracking protein aggregation and mislocalization in cells with flow cytometry Nature Methods doi:10.1038/nmeth.1930 [HSPA1A]

Histone deacetylase inhibitors prevent the degradation and restore the activity of glucocerebrosidase in Gaucher disease PNAS, Dec 2011; 108: 21200 - 21205. [anti-DDK antibody]

Novel p65 Binding Glucocorticoid-induced Leucine Zipper Peptide Suppresses Experimental Autoimmune Encephalomyelitis J. Biol. Chem., Dec 2011; 286: 44799 - 44810. [anti-DDK]

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5A2 anti-eGFP Monoclonal Antibody

Anti-tGFP Western Blot Image
Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA50052) (1:2000).

The 5A2 anti-eGFP (enhanced green fluorescent protein) monoclonal antibody has been developed for the detection of eGFP tag fused to the N- or C-terminus of recombinant proteins

  • Validated for Western blot
  • High specificity and sensitivity
  • Detect recombinant proteins with low expression levels

Immunogen:

A full length eGFP tagged recombinant protein expressed in HEK293T cell

eGFP (enhanced green fluorescent protein):

eGFP is an improved variant of GFP (green fluorescent protein) with intense green fluorescence. It is an ideal tool for monitoring the dynamics of tagged proteins for protein-protein integration, cell-sorting, organelle labeling, protein localization, gene expression and more.

SKU Description Data Sheet Price Shipping  
TA150052 5A2, Anti-eGFP mouse monoclonal antibody (100 µL) pdf $248 Immediate Add to Shopping Cart

* For bulk antibody package, please contact Tech Support

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Western Blot Protocol


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