Home Antibody Antitag Antibodies Anti-eGFP 5A2
5A2 anti-eGFP Monoclonal Antibody
Figure 1. HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA50052) (1:2000).
The 5A2 anti-eGFP (enhanced green fluorescent protein) monoclonal antibody has been developed for the detection of eGFP tag fused to the N- or C-terminus of recombinant proteins
- Validated for Western blot
- High specificity and sensitivity
- Detect recombinant proteins with low expression levels
A full length eGFP tagged recombinant protein expressed in HEK293T cell
eGFP (enhanced green fluorescent protein):
eGFP is an improved variant of GFP (green fluorescent protein) with intense green fluorescence. It is an ideal tool for monitoring the dynamics of tagged proteins for protein-protein integration, cell-sorting, organelle labeling, protein localization, gene expression and more.
||5A2, Anti-eGFP mouse monoclonal antibody (100 µL)