OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right

Video overview of cDNA clone offering
Video brochure for cDNA clone
Related Products

Over 1000 citations of OriGene cDNA clones
Chimeric Proteins Combining Phosphatase and Cellulose-Binding Activities: Proof-of-Concept and Application in the Hydrolysis of Paraoxon Protein Pept. Lett. May 2014 [PON1]

POT1 loss-of-function variants predispose to familial melanoma Nat. Genet. 478-81 46 5. May 2014 [POT1]

18ß-Glycyrrhetinic Acid Suppresses Cell Proliferation through Inhibiting Thromboxane Synthase in Non-Small Cell Lung Cancer PLoS ONE e93690 9 4. April 2014 [TxAS]

A Bacteriophage Capsid Protein Provides a General Amyloid Interaction Motif (GAIM) That Binds and Remodels Misfolded Protein Assemblies J. Mol. Biol. April 2014 [SCNA]

View All Citations >>

 

LGR5 (NM_003667) Stable Cell Line

Catalog #:SL500063Price:$12888    technical questions?
Description:Human LGR5 Stable Cell Line - NIH3T3
Gene:LGR5
Other names: FEX; GPR49; GPR67; GRP49; HG38
Host CellNIH3T3
cDNA Clone:
Format:Cryopreserved cells, 4 x 10E6 cells/vial
Mycoplasma:Negative by MycoAlert Kit, Lonza
Cell Line Validation:Western blot and Flow cytometry
Background:Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), also known as GPR49, is a member of 7-transmembrane orphan receptor. Although its ligand and function are unclear, LGR5 has been reported as a target of Wnt signaling pathway and a stem cell marker of small intestine and colon. Recent studies clearly show functional correlations between LGR5 expression and tumorgenesis in ovarian and liver cancer, especially in colorectal cancer (CRC). LGR5 is involved in both early events and late events in colorectal tumorgenesis. Moreover, LGR5 expression is higher in colon cancer cell lines derived from metastatic tumors compared with those from primary tumors. The over expression of LGR5 is significantly correlated with lymphatic invasion, vascular invasion, tumor depth, lymph node metastasis and tumor stage. LGR5 is an important biomarker in cancer diagnosis and treatment.
Figure 1. Western blot analysis for NIH3T3/LGR5 using OriGene mAb clone 2A2 (Cat# TA503316) against human LGR5. 15 ug of membrane protein was used for this Western blot analysis. Membrane protein concentration was measured by BCA kit (Thermo Scientific Inc.). Lane A: membrane protein from NIH3T3/LGR5 stable cell line with the specific band around 100K of LGR5. Lane B: membrane protein from NIH3T3 cells as negative control.
Figure 2. Flow Cytometry Analysis: Black: NIH3T3 cells incubated with LGR5 mAb 2A2 Red: NIH3T3/LGR5 stable cell line incubated with LGR5 mAb 2A2

Related Products

 

patter
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title