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Home Antibody Secondary Antibodies

Goat Anti-Mouse Secondary Antibody

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SKU Description Datasheet Price Availability*  
TA140002
  • Peroxidase Conjugated Goat Anti-Mouse IgG (Min Hu, Rb), 1mg
$100 In stock Add to Shopping Cart

Specification


Product NamePeroxidase Conjugated Goat Anti-Mouse IgG (Min Hu, Rb)
TargetMouse IgG
ImmunogenMouse IgG, whole molecule
ConjugationHRP
ConcentrationN/A (lyophilized)
Host SpeciesGoat
IsoTypeIgG
ApplicationWB: 1:3,000-10,000; ELISA: 1:20,000-40,000
FormLyophilized powder
Buffer0.01M PBS (pH7.4)
PurificationThis antibody was purified from antiserum by immunoaffinity chromatography which removes essentially all goat serum proteins, except the specific antibody for mouse IgG.
StorageStore lyophilized powders at 2-8C. When ready to use, reconstitute with 0.5ml dH2O and centrifuge briefly. Product is stable at 2-8C for 6 weeks as undiluted liquid. Prepare small aliquots to avoid repeated freezing and thawing. For extended storage after rehydration, aliquot and store at -20C or below. Product will be state for one year after rehydration. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50% and store at -20C. Please note that, adding glycerol reduces the stated protein concentration by half and the dilution ratio should be adjusted accordingly.
NoteThis peroxidase conjugated antibody is specific for mouese IgG and shows no cross-reactivity other animal species IgG proteins.
WB Image
West blot analysis of GAPDH expression in 9 cell lines using mouse anti-GAPDH as primary antibody and HRP conjugated goat anti-mouse IgG as secondary antibody (TA140002).
ELISA Image
Comparison of background activities between OriGene (OTI) HRP-conjugated goat anti-mouse against human IgG (TA140002) and a comparable antibody from Company J.
ELISA Image
Comparison of background activities between OriGene (OTI) HRP-conjugated goat anti-mouse against rabbit IgG (TA140002) and a comparable antibody from Company J.
ELISA Image
ELISA analysis of mouse IgG with HRP-conjugated goat anti-mouse secondary (TA140002) or with a comparable product from Company J.
ELISA Image
ELISA analysis of DDK (FLAG tag) with different dilutions of mouse anti-DDK primary antibody and anti-mouse secondary antibody. 1) Dark Green: OriGene (OTI) HRP-conjugated goat anti-mouse secondary (TA140002 diluted at 1:20,000); 2) Light Green: TA140002 diluted at 1:40,000; 3) Red: a comparable secondary antibody from Company J.

 

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