Aatf (NM_053720) Rat Untagged Clone

CAT#: RN203248

Aatf (untagged ORF) - Rat apoptosis antagonizing transcription factor (Aatf), (10 ug)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Aatf
• Synonyms: Ded
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN203248 representing NM_053720
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGCAGCCCCTGGCTCTGCAGCTGGAACAGTTGTTGAATCCAAGACCGCGTGAGGCGGATC
  CTGAGGCGGACCCGGAGGAGGCCACTAGAGCCAGAGTGATTGACAGGTTTGATGAAGGGGAAGAGGAAGA
  AGATGATCTGCCAGTGAGTAGTATTAGAAAGTTGGCACCGGTCTCCCTCTTGGACACCGACAAAAGGTAT
  TCTGGCAAGACCACTTCTCGGAAAGCTTGGAAAGAAGACCATTGGGACCAGACTCTGCCAAGTTCATCGG
  ACAATGAAATACCTGATGAAGGAGGCTCTGAAGCTGGGGATTCAGAGGGCCTGGAGGAGCTCTCAGAAGA
  TGTTGAGGAAGACTTGGAAGACAATGAAATACCTGATGAAGGAGGCTCTGAAGATGGGGATTCAGAGGGC
  CTGGAGGAGGAGATCTCAGAAGATGTTGAGGAAGACTTAGAAGGTGAGGATGAGGAAGACAGAGAAGAGG
  ACAGGAACAGTGAAGATGACGGCGTGGTGATGGCCTTCTCCGGTGTTAAAGTTTCTGAGGAAGTGGAAAA
  AGGAAGAGCTGTGAAGAACCAGATAGCGTTGTGGGACCAGCTCTTGGAAGGAAGGATCAAACTACAAAAA
  GCTCTCTTGACTACCAATCAATTGCCTCAACCAGATGTTTTCCCTGTCTTCAAGGACAAAGGTGGCCCAG
  AATTTGCCAGTGCTCTGAAAAATAGTCACAAAGCACTTAAAGCATTGTTAAGATCATTGGTGGATCTTCA
  GGAAGAGTTGCTTTTCCAGTACCCAGATACAAGATATTTAGTAAAAGGGACGAAACCCAATGCAGAGAGT
  GAGGAGATTTCCAGTGAAGATGATGAGCTAGTGGGAGAGAAGAAGAAACAAAGAAAGGCCCCACCAAAGA
  GGAAGCTAGAGATGGAGGACTATCCCAGCTTCATGGCAAAGCGCTCTGCTGACTTTACCGTCTACAGGAA
  CCGCACACTACAAAAATGGCATGATAAAACCAAGCTTGCTTCTGGAAAACTGGGCAAGGGTTTCGGTGCC
  TTTGAACGCTCCATCTTGACTCAGATTGATCATATTCTGATGGACAAAGAAAGATTACTTCGAAGGACGC
  AGACCAAGCGCTCTGCCTACCGAGTTCTTGGCAAACCTGAGCCAGTCCCTGAGCCTGTTGCAGAGACTTT
  GCCAGGAGAACCGGAGAGCCTTCCTCAGGTCCCTGCCAATGCTCACCTGAAGGACTTGGACGAGGAGATA
  TTTGATGATGATGACTTCTACCACCAGCTCCTTCGAGAACTCATAGAGCGGAAGACCAGCTCTTTGGATC
  CAAATGATCAGGTGGCCATGGGAAGGCAGTGGCTTGCAATCCAGAAACTACGAAGCAAAATCCGCAAGAA
  GGTAGATAGAAAAGCCAGCAAAGGAAGAAAACTTCGGTTCCATGTGCTTAGCAAGCTCCTGAGTTTCATG
  GCGCCTATTGACCACACTGCAATGAATGATGAGGCCAGGACCGAGTTGTATCGATCTCTTTTCGGCCAGC
  TCAACCGTCTGGACGCAGACCATGGGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_053720
• Insert Size: 1572 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053720.1, NP_446172.1
• RefSeq Size: 1805 bp
• RefSeq ORF: 1572 bp
• Locus ID: 114512
• UniProt ID: Q9QYW0
• Cytogenetics: 10q26
• Gene Summary: an apoptosis antagonizing nuclear phosphoprotein transcription factor [RGD, Feb 2006]