Aadat (NM_017193) Rat Tagged ORF Clone

CAT#: RR213490

Aadat (Myc-DDK-tagged ORF) - Rat aminoadipate aminotransferase (Aadat), (10 ug)

Product Images

Circular map for RR213490

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Aadat
• Synonyms: Kat2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR213490 representing NM_017193
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATTACTCAAGGTTCCTCACTGCAACGAGCCTGGCCAGAAAGACATCCCCTATCAGAGCTACAGTGG
  AGATAATGAGTAGAGCACCCAAAGACATCATCTCCCTGGCTCCTGGATCTCCGAACCCGAAAGTGTTCCC
  CTTTAAGTCAGCTGTCTTCACTGTGGAGAACGGAAGCACCATCCGGTTTGAAGGAGAGATGTTTCAAAGG
  GCCCTCCAATATTCCTCAAGCTATGGAATTCCAGAACTTCTGTCCTGGCTAAAACAGTTGCAAATAAAAT
  TGCATAATCCTCCGACTGTCAACTACTCACCCAACGAAGGACAGATGGACCTCTGCATCACATCTGGCTG
  TCAAGACGGTCTCTGTAAGGTGTTTGAAATGCTCATCAATCCTGGAGACACTGTTCTGGTCAATGAACCA
  CTGTATTCAGGAGCCCTTTTTGCAATGAAACCACTGGGCTGCAATTTTATTAGTGTCCCCAGTGATGACT
  GTGGGATTATTCCAGAGGGTCTCAAAAAAGTACTTTCCCAGTGGAAACCAGAAGATTCCAAGGATCCCAC
  AAAAAGGACTCCAAAATTTCTGTATACTATTCCGAATGGCAACAACCCTACAGGCAACTCGTTGACTGGT
  GACCGCAAGAAAGAAATCTATGAGCTTGCAAGAAAATATGACTTCCTCATAATAGAAGACGATCCTTACT
  ATTTTCTCCAGTTCACCAAGCCTTGGGAACCAACCTTTCTCTCCATGGATGTTGATGGGAGAGTTATCAG
  AGCTGACTCCCTTTCAAAAGTTATCTCCTCAGGGCTGAGAGTGGGGTTTATAACTGGCCCCAAGTCCTTG
  ATACAGAGGATTGTTCTCCACACACAAATCTCATCACTGCATCCCTGTACTTTATCACAGCTCATGATAT
  CGGAGCTTCTATACCAGTGGGGAGAAGAGGGTTTCCTGGCCCATGTTGACAGAGCTATTGATTTCTACAA
  GAACCAGAGGGATTTTATATTGGCAGCTGCAGACAAGTGGTTACGTGGTTTGGCAGAGTGGCATGTTCCC
  AAAGCTGGCATGTTTCTATGGATTAAAGTTAACGGAATCTCTGATGCAAAAAAACTAATTGAAGAAAAGG
  CTATTGAAAGAGAGATCTTGTTAGTTCCTGGAAATAGTTTCTTCGTCGATAATTCAGCCCCCTCCTCCTT
  CTTCAGAGCATCCTTCTCTCAGGTTACTCCAGCGCAGATGGACTTAGTCTTCCAGAGATTGGCCCAACTC
  ATAAAAGACGTTTCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR213490 representing NM_017193
  Red=Cloning site Green=Tags(s)
  
  MNYSRFLTATSLARKTSPIRATVEIMSRAPKDIISLAPGSPNPKVFPFKSAVFTVENGSTIRFEGEMFQR
  ALQYSSSYGIPELLSWLKQLQIKLHNPPTVNYSPNEGQMDLCITSGCQDGLCKVFEMLINPGDTVLVNEP
  LYSGALFAMKPLGCNFISVPSDDCGIIPEGLKKVLSQWKPEDSKDPTKRTPKFLYTIPNGNNPTGNSLTG
  DRKKEIYELARKYDFLIIEDDPYYFLQFTKPWEPTFLSMDVDGRVIRADSLSKVISSGLRVGFITGPKSL
  IQRIVLHTQISSLHPCTLSQLMISELLYQWGEEGFLAHVDRAIDFYKNQRDFILAAADKWLRGLAEWHVP
  KAGMFLWIKVNGISDAKKLIEEKAIEREILLVPGNSFFVDNSAPSSFFRASFSQVTPAQMDLVFQRLAQL
  IKDVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017193
• ORF Size: 1275 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017193.1, NP_058889.1
• RefSeq Size: 1828 bp
• RefSeq ORF: 1278 bp
• Locus ID: 29416
• UniProt ID: Q64602
• Cytogenetics: 16p12
• MW: 47.8 kDa
• Gene Summary: endogenous modulator of glutamatergic neurotransmission with kynurenine aminotransferase (KAT) activity [RGD, Feb 2006]