OriGene - Gad1 (NM_017007) Human ORF cDNA Clone

Western Blot Validated

TrueORF Data for RR212955

Vector:	pCMV6-Entry Change vector?	Tag: C-terminal Myc-DDK	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS	ORF Size: 1782 bp Predicted Protein MW: kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:
Protein Pathways:

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_017007.1, NP_058703	RefSeq Size: 3216	RefSeq ORF: 1782
Synonyms : Gad67
LocusID: 24379	Cytogenetic: 3q21
Summary: plays a role in the biosynthesis of gamma-aminobutyric acid [RGD, Feb 2006].

ORF Clones Citations:

Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis, Svenja Meiler, Yvonne Baumer, Zhi Huang, Fukun W. Hoffmann, Gregory J. Fredericks, Aaron H. Rose, Robert L. Norton, Peter R. Hoffmann, and William A. Boisvert, J. Leukoc. Biol., May 2013; 93: 771 - 780. [CD36]

Trafficking of the Transcription Factor Nrf2 to Promyelocytic Leukemia-Nuclear Bodies: IMPLICATIONS FOR DEGRADATION OF NRF2 IN THE NUCLEUS, Melanie Theodore Malloy, Deneshia J. McIntosh, Treniqka S. Walters, Andrea Flores, J. Shawn Goodwin, and Ifeanyi J. Arinze, J. Biol. Chem., May 2013; 288: 14569 - 14583. [RNF4]

Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle, Paulo A. Gameiro, Laura A. Laviolette, Joanne K. Kelleher, Othon Iliopoulos, and Gregory Stephanopoulos, J. Biol. Chem., May 2013; 288: 12967 - 12977. [NNT]

Inverted Formin 2 Regulates Actin Dynamics by Antagonizing Rho/Diaphanous-related Formin Signaling, Hua Sun, Johannes Schlondorff, Henry N. Higgs, and Martin R. Pollak, J. Am. Soc. Nephrol., May 2013; 24: 917 - 929. [INF2]

DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer, Junying Wang, Limei Sun, Mingyue Yang, Wenting Luo, Ying Gao, Zihui Liu, Xueshan Qiu, and Enhua Wang, Journal of Histochemistry & Cytochemistry, May 2013; 10.1369/0022155413488120. [DEK]

A model of lysosomal pH regulation, Yoichi Ishida, Smita Nayak, Joseph A. Mindell, and Michael Grabe, J. Gen. Physiol., May 2013; 141: 705 - 720. [CLCN7]

A model of lysosomal pH regulation, Yoichi Ishida, Smita Nayak, Joseph A. Mindell, and Michael Grabe, J. Gen. Physiol., May 2013; 141: 705 - 720. [OSTM1]

The Serum- and Glucocorticoid-inducible Kinases SGK1 and SGK3 Regulate hERG Channel Expression via Ubiquitin Ligase Nedd4-2 and GTPase Rab11, Shawn M. Lamothe and Shetuan Zhang, J. Biol. Chem., May 2013; 288: 15075 - 15084. [SGK1]

Regulation of the Bone-restricted IFITM-like (Bril) Gene Transcription by Sp and Gli Family Members and CpG Methylation, Bahar Kasaai, Marie-Hélène Gaumond, and Pierre Moffatt, J. Biol. Chem., May 2013; 288: 13278 - 13294. [SP3]

More TrueClone Citations >>

Gad1 Human Clones

SKU	Description	Amount	Price	Shipping
SC115217	Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD25 as transfection-ready DNA	10 ug	$380	Next day
SC119631	Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as transfection-ready DNA	10 ug	$185	Next day
RC216201	Myc-DDK-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD25 as transfection-ready DNA	10 ug	$380	3 weeks
RC207226	Myc-DDK-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as transfection-ready DNA	10 ug	$540	Next day
RC207226L1	Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 , Myc-DDK-tagged	10 ug	$910	2 weeks
RG207226	GFP-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as transfection-ready DNA	10 ug	$590	Next day
RC207226L2	Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67, mGFP tagged	10 ug	$1000	2 weeks
RG216201	GFP-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD25 as transfection-ready DNA	10 ug	$420	3 weeks
RC507226	Myc-DDK-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as 200 ug transfection-ready DNA	10 ug	$660	2~3 weeks weeks

Gad1 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC201947	Mus musculus glutamic acid decarboxylase 1 (Gad1) as transfection-ready DNA	10 ug	$165	Next day
MR209243	Myc-DDK-tagged ORF clone of Mus musculus glutamic acid decarboxylase 1 (Gad1) as transfection-ready DNA	10 ug	$710	Next day
MG209243	GFP-tagged ORF clone of Mus musculus glutamic acid decarboxylase 1 (Gad1) in a GFP expression vector	10 ug	$780	Next day

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Gad1 (NM_017007) Rat cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

Gad1 Human Clones

Gad1 Mouse Clones

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