OriGene - Ech1 (NM_022594) Human ORF cDNA Clone

Western Blot Validated

TrueORF Data for RR210474

Vector:	pCMV6-Entry Change vector?	Tag: C-terminal Myc-DDK	Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Sequence Data:	ORF Nucleotide Sequence CHROMATOGRAMS	ORF Size: 984 bp Predicted Protein MW: kDa
Restriction Sites:	SgfI-MluI Cloning Scheme for this gene Plasmid Map
OTI Annotation:	This clone was engineered to express the complete ORF with an expression tag.
OTI Disclaimer:	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components:	The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Protein Families:
Protein Pathways:

* The lysates used for this WB picture contain the overexpressed empty vector or the Myc-DDK tagged ORF clone.

Reference Data

RefSeq: NM_022594.1, NP_072116	RefSeq Size: 1097	RefSeq ORF: 984
Synonyms : Pxel
LocusID: 64526	Cytogenetic: 1q21
Summary: isomerase enzyme that may be involved with the ability of peroxisomes to oxidize long-chain faty acids [RGD, Feb 2006].

ORF Clones Citations:

Selenoprotein K is required for palmitoylation of CD36 in macrophages: implications in foam cell formation and atherogenesis, Svenja Meiler, Yvonne Baumer, Zhi Huang, Fukun W. Hoffmann, Gregory J. Fredericks, Aaron H. Rose, Robert L. Norton, Peter R. Hoffmann, and William A. Boisvert, J. Leukoc. Biol., May 2013; 93: 771 - 780. [CD36]

Trafficking of the Transcription Factor Nrf2 to Promyelocytic Leukemia-Nuclear Bodies: IMPLICATIONS FOR DEGRADATION OF NRF2 IN THE NUCLEUS, Melanie Theodore Malloy, Deneshia J. McIntosh, Treniqka S. Walters, Andrea Flores, J. Shawn Goodwin, and Ifeanyi J. Arinze, J. Biol. Chem., May 2013; 288: 14569 - 14583. [RNF4]

Cofactor Balance by Nicotinamide Nucleotide Transhydrogenase (NNT) Coordinates Reductive Carboxylation and Glucose Catabolism in the Tricarboxylic Acid (TCA) Cycle, Paulo A. Gameiro, Laura A. Laviolette, Joanne K. Kelleher, Othon Iliopoulos, and Gregory Stephanopoulos, J. Biol. Chem., May 2013; 288: 12967 - 12977. [NNT]

Inverted Formin 2 Regulates Actin Dynamics by Antagonizing Rho/Diaphanous-related Formin Signaling, Hua Sun, Johannes Schlondorff, Henry N. Higgs, and Martin R. Pollak, J. Am. Soc. Nephrol., May 2013; 24: 917 - 929. [INF2]

DEK Depletion Negatively Regulates Rho/ROCK/MLC Pathway in Non–Small Cell Lung Cancer, Junying Wang, Limei Sun, Mingyue Yang, Wenting Luo, Ying Gao, Zihui Liu, Xueshan Qiu, and Enhua Wang, Journal of Histochemistry & Cytochemistry, May 2013; 10.1369/0022155413488120. [DEK]

A model of lysosomal pH regulation, Yoichi Ishida, Smita Nayak, Joseph A. Mindell, and Michael Grabe, J. Gen. Physiol., May 2013; 141: 705 - 720. [CLCN7]

A model of lysosomal pH regulation, Yoichi Ishida, Smita Nayak, Joseph A. Mindell, and Michael Grabe, J. Gen. Physiol., May 2013; 141: 705 - 720. [OSTM1]

The Serum- and Glucocorticoid-inducible Kinases SGK1 and SGK3 Regulate hERG Channel Expression via Ubiquitin Ligase Nedd4-2 and GTPase Rab11, Shawn M. Lamothe and Shetuan Zhang, J. Biol. Chem., May 2013; 288: 15075 - 15084. [SGK1]

Regulation of the Bone-restricted IFITM-like (Bril) Gene Transcription by Sp and Gli Family Members and CpG Methylation, Bahar Kasaai, Marie-Hélène Gaumond, and Pierre Moffatt, J. Biol. Chem., May 2013; 288: 13278 - 13294. [SP3]

More TrueClone Citations >>

Ech1 Human Clones

SKU	Description	Amount	Price	Shipping
SC126175	Homo sapiens enoyl Coenzyme A hydratase 1, peroxisomal, mRNA (cDNA clone MGC:19561 IMAGE:4300082), complete cds as transfection-ready DNA	10 ug	$270	3 weeks
SC119261	Homo sapiens enoyl CoA hydratase 1, peroxisomal (ECH1) as transfection-ready DNA	10 ug	$580	Next day
RC205622	Myc-DDK-tagged ORF clone of Homo sapiens enoyl CoA hydratase 1, peroxisomal (ECH1) as transfection-ready DNA	10 ug	$460	Next day
RC205622L1	Lenti ORF clone of Human enoyl CoA hydratase 1, peroxisomal (ECH1) , Myc-DDK-tagged	10 ug	$830	2 weeks
RC205622L2	Lenti ORF clone of Human enoyl CoA hydratase 1, peroxisomal (ECH1), mGFP tagged	10 ug	$910	2 weeks
RG205622	GFP-tagged ORF clone of Homo sapiens enoyl CoA hydratase 1, peroxisomal (ECH1) as transfection-ready DNA	10 ug	$510	Next day
RC505622	Myc-DDK-tagged ORF clone of Homo sapiens enoyl CoA hydratase 1, peroxisomal (ECH1) as 200 ug transfection-ready DNA	10 ug	$580	2~3 weeks weeks

Ech1 Mouse Clones

SKU	Description	Amount	Price	Shipping
MC206124	Mus musculus enoyl coenzyme A hydratase 1, peroxisomal (cDNA clone MGC:92936 IMAGE:5710971) as transfection-ready DNA	10 ug	$165	Next day
MR204801	Myc-DDK-tagged ORF clone of Mus musculus enoyl coenzyme A hydratase 1, peroxisomal (Ech1) as transfection-ready DNA	10 ug	$380	Next day
MG204801	GFP-tagged ORF clone of Mus musculus enoyl coenzyme A hydratase 1, peroxisomal (Ech1) in a GFP expression vector	10 ug	$420	3 weeks

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Ech1 (NM_022594) Rat cDNA ORF Clone

Western Blot Validated

ORF Clones Citations:

Ech1 Human Clones

Ech1 Mouse Clones

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