CYP7A1 (NM_000780) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC211019. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 57.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: ES Cell Differentiation/IPSTransmembraneP450Druggable Genome Protein Pathways: Primary bile acid biosynthesisMetabolic pathwaysPPAR signaling pathway

Protein Data

RefSeq: NP_000771	RefSeq Size: 2877	RefSeq ORF: 1515
LocusID: 1581	Cytogenetic: 8q11
Synonyms : CP7A; CYP7; CYPVII
Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) as transfection-ready DNA • Lenti ORF clone of Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), mGFP tagged • Lenti ORF clone of Human cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CYP7A1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CYP7A1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Cyp7a1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CYP7A1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Cyp7a1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CYP7A1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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