MTNR1A (NM_005958) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC210385. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 39.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: GPCRTransmembraneDruggable Genome Protein Pathways: Neuroactive ligand-receptor interaction

Protein Data

RefSeq: NP_005949	RefSeq Size: 1105	RefSeq ORF: 1053
LocusID: 4543	Cytogenetic: 4q35.1
Synonyms : MEL-1A-R; MT1
Summary: This gene encodes one of two high affinity forms of a receptor for melatonin, the primary hormone secreted by the pineal gland. This receptor is a G-protein coupled, 7-transmembrane receptor that is responsible for melatonin effects on mammalian circadian rhythm and reproductive alterations affected by day length. The receptor is an integral membrane protein that is readily detectable and localized to two specific regions of the brain. The hypothalamic suprachiasmatic nucleus appears to be involved in circadian rhythm while the hypophysial pars tuberalis may be responsible for the reproductive effects of melatonin. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens melatonin receptor 1A (MTNR1A) as transfection-ready DNA • Lenti ORF clone of Human melatonin receptor 1A (MTNR1A) , Myc-DDK-tagged • Lenti ORF clone of Human melatonin receptor 1A (MTNR1A), mGFP tagged • GFP-tagged ORF clone of Homo sapiens melatonin receptor 1A (MTNR1A) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens melatonin receptor 1A (MTNR1A) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of melatonin receptor 1A (MTNR1A) Validated Antibodies • Rabbit polyclonal antibody to MEL-1A-R (melatonin receptor 1A) • Goat Anti-MTNR1A Antibody • Rabbit polyclonal anti-MTR1A antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • MTNR1A - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • MTNR1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Mtnr1a - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • MTNR1A - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Mtnr1a - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene MTNR1A

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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