RBPJ (NM_203284) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC209094. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 54.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transcription Factors Protein Pathways: Notch signaling pathway

Protein Data

RefSeq: NP_976029	RefSeq Size: 2499	RefSeq ORF: 1461
LocusID: 3516	Cytogenetic: 4p15.2
Synonyms : AOS3; CBF1; csl; IGKJRB; IGKJRB1; KBF2; RBP-J; RBPJK; RBPSUH; SUH
Summary: The protein encoded by this gene is a transcriptional regulator important in the Notch signaling pathway. The encoded protein acts as a repressor when not bound to Notch proteins and an activator when bound to Notch proteins. It is thought to function by recruiting chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins to Notch signaling pathway genes. Also, this protein can bind specifically to the recombination signal sequence of immunglobulin kappa type J segments. Several transcript variants encoding different isoforms have been found for this gene, and several pseudogenes of this gene exist on chromosome 9. [provided by RefSeq, Oct 2011].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4 as transfection-ready DNA • Lenti ORF clone of Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4 , Myc-DDK-tagged • Lenti ORF clone of Human recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4, mGFP tagged • GFP-tagged ORF clone of Homo sapiens recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of recombination signal binding protein for immunoglobulin kappa J region (RBPJ), transcript variant 4 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • RBPJ - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • RBPJ - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Rbpj - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • RBPJ - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Rbpj - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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