F10 (NM_000504) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC208506. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 52.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: TransmembraneProteaseDruggable Genome Protein Pathways: Complement and coagulation cascades

Protein Data

RefSeq: NP_000495	RefSeq Size: 1560	RefSeq ORF: 1467
LocusID: 2159	Cytogenetic: 13q34
Synonyms : FX; FXA
Summary: This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens coagulation factor X (F10) as transfection-ready DNA • Lenti ORF clone of Human coagulation factor X (F10) , Myc-DDK-tagged • Lenti ORF clone of Human coagulation factor X (F10), mGFP tagged • GFP-tagged ORF clone of Homo sapiens coagulation factor X (F10) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens coagulation factor X (F10) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of coagulation factor X (F10) Validated Antibodies • Rabbit Polyclonal antibody to Factor X (coagulation factor X) • Rabbit polyclonal FA10 (activated heavy chain, Cleaved-Ile235) antibody • Rabbit polyclonal FA10 (light chain, Cleaved-Ala41) antibody • Anti-DR3 Rabbit Polyclonal Antibody • Anti-TLR9 Rabbit Polyclonal Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • F10 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • F10 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • F10 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • F10 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • F10 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene F10

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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