AXIN1 (NM_181050) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC208349. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 91.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell relevant signaling - Wnt Signaling pathwayES Cell Differentiation/IPSDruggable Genome Protein Pathways: Wnt signaling pathwayPathways in cancerColorectal cancerEndometrial cancerBasal cell carcinoma

Protein Data

RefSeq: NP_851393	RefSeq Size: 3369	RefSeq ORF: 2481
LocusID: 8312	Cytogenetic: 16p13.3
Synonyms : AXIN; PPP1R49
Summary: This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli, catenin beta-1, glycogen synthase kinase 3 beta, protein phosphate 2, and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family, member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein, alone and in a complex with other proteins, has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma, hepatoblastomas, ovarian endometriod adenocarcinomas, and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2010].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens axin 1 (AXIN1), transcript variant 2 as transfection-ready DNA • Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 2 , Myc-DDK-tagged • Lenti ORF clone of Human axin 1 (AXIN1), transcript variant 2, mGFP tagged • GFP-tagged ORF clone of Homo sapiens axin 1 (AXIN1), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens axin 1 (AXIN1), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of axin 1 (AXIN1), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • AXIN1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • AXIN1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Axin1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • AXIN1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Axin1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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