BID (NM_001196) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC207261. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 21.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: p53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseAmyotrophic lateral sclerosis (ALS)Pathways in cancerMore Pathways >>

Protein Data

RefSeq: NP_001187	RefSeq Size: 2214	RefSeq ORF: 588
LocusID: 637	Cytogenetic: 22q11.1
Synonyms : FP497
Summary: This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens BH3 interacting domain death agonist (BID), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of BH3 interacting domain death agonist (BID), transcript variant 2 Validated Antibodies • Rabbit Polyclonal Bid Antibody • Rabbit Polyclonal Bid Antibody • Purified BID mouse monoclonal antibody,clone 2B6 • Purified BID mouse monoclonal antibody,clone 1G10 • Purified BID mouse monoclonal antibody,clone 2H9 • Purified BID mouse monoclonal antibody,clone 4A1 • Purified BID mouse monoclonal antibody,clone 3E6 • Purified BID mouse monoclonal antibody,clone 2A2 • Purified BID mouse monoclonal antibody,clone 1G1 • Purified BID mouse monoclonal antibody,clone 3E9 • Purified BID mouse monoclonal antibody,clone 3A10 • Purified BID mouse monoclonal antibody,clone 3C8 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 2B6 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 1G10 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 4A1 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 3E6 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 2A2 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 1G1 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 3E9 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 3A10 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 3C8 • Carrier-free (BSA/glycerol-free) BID mouse monoclonal antibody,clone 2H9 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • BID - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • BID - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Bid - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • BID - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Bid - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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