GAD1 (NM_000817) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC207226. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 66.7 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Alanine, aspartate and glutamate metabolismbeta-Alanine metabolismTaurine and hypotaurine metabolismButanoate metabolismMetabolic pathwaysType I diabetes mellitus

Protein Data

RefSeq: NP_000808	RefSeq Size: 3488	RefSeq ORF: 1785
LocusID: 2571	Cytogenetic: 2q31
Synonyms : CPSQ1; GAD; SCP
Summary: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as transfection-ready DNA • Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 , Myc-DDK-tagged • Lenti ORF clone of Human glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of glutamate decarboxylase 1 (brain, 67kDa) (GAD1), transcript variant GAD67 Validated Antibodies • Rabbit polyclonal anti-GAD67/GAD1 antibody • Purified GAD1 (GAD67) mouse monoclonal antibody, clone 3H2 • Purified GAD1 (GAD67) mouse monoclonal antibody, clone 3G9 • Purified anti-GAD1 (GAD67) mouse monoclonal antibody, clone 5D8 • Goat Anti-GAD1 (isoform GAD67) Antibody • Rabbit monoclonal antibody against GAD1(clone EPR2550) • Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody, clone 3H2 • Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody, clone 3G9 • Carrier-free (BSA/glycerol-free) GAD1 mouse monoclonal antibody,clone 5D8 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • GAD1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • GAD1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Gad1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • GAD1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Gad1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene GAD1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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