IL3RA (NM_002183) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC206420. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 41.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transmembrane Protein Pathways: Cytokine-cytokine receptor interactionApoptosisJak-STAT signaling pathwayHematopoietic cell lineage

Protein Data

RefSeq: NP_002174	RefSeq Size: 1726	RefSeq ORF: 1137
LocusID: 3563	Cytogenetic: Xp22.3
Synonyms : CD123; hIL-3Ra; IL3R; IL3RAY; IL3RX; IL3RY
Summary: The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens interleukin 3 receptor, alpha (low affinity) (IL3RA) as transfection-ready DNA • Lenti ORF clone of Human interleukin 3 receptor, alpha (low affinity) (IL3RA) , Myc-DDK-tagged • Lenti ORF clone of Human interleukin 3 receptor, alpha (low affinity) (IL3RA), mGFP tagged • GFP-tagged ORF clone of Homo sapiens interleukin 3 receptor, alpha (low affinity) (IL3RA) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens interleukin 3 receptor, alpha (low affinity) (IL3RA) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of interleukin 3 receptor, alpha (low affinity) (IL3RA) Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • IL3RA - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • IL3RA - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Il3ra - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • IL3RA - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Il3ra - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene IL3RA

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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