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GPT (NM_005309) Purified Human Protein

Specifications Citations (1) Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP303756 Recombinant protein of human glutamic-pyruvate transaminase (alanine aminotransferase) (GPT), 20 ug (Larger pack size?) $680 In Stock
TA50011-100 Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody $248 In Stock
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC203756. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:54.5 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families: Druggable Genome
Protein Pathways: Alanine, aspartate and glutamate metabolismMetabolic pathways
Protein Data
Protein Image

Reference Data
RefSeq: NP_005300 RefSeq Size: 1924 RefSeq ORF: 1491
LocusID: 2875 Cytogenetic: 8q24.3  
Synonyms : AAT1; ALT1; GPT1
Summary: This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009].
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich


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