BCKDK (NM_005881) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203601. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 46.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways:

Protein Data

RefSeq: NP_005872	RefSeq Size: 1890	RefSeq ORF: 1239
LocusID: 10295	Cytogenetic: 16p11.2
Synonyms : BCKDKD; BDK
Summary: The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1 Validated Antibodies • Purified Rabbit Polyclonal Antibody against BCKDK (Center) • Purified BCKDK (BCKDH kinase) mouse monoclonal antibody, clone 11C9 • Carrier-free (BSA/glycerol-free) BCKDK mouse monoclonal antibody, clone 11C9 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • BCKDK - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • BCKDK - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Bckdk - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • BCKDK - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Bckdk - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene BCKDK

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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