TPM3 (NM_152263) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203276. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 32.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein Pathways: Cardiac muscle contractionPathways in cancerThyroid cancerHypertrophic cardiomyopathy (HCM)Dilated cardiomyopathy

Protein Data

RefSeq: NP_689476	RefSeq Size: 7116	RefSeq ORF: 858
LocusID: 7170	Cytogenetic: 1q21.2
Synonyms : CFTD; hscp30; hTM5; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TM5; TPMsk3; TRK
Summary: This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens tropomyosin 3 (TPM3), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human tropomyosin 3 (TPM3), transcript variant 1 , Myc-DDK-tagged • GFP-tagged ORF clone of Homo sapiens tropomyosin 3 (TPM3), transcript variant 1 as transfection-ready DNA • Lenti ORF clone of Human tropomyosin 3 (TPM3), transcript variant 1, mGFP tagged • Myc-DDK-tagged ORF clone of Homo sapiens tropomyosin 3 (TPM3), transcript variant 1 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of tropomyosin 3 (TPM3), transcript variant 1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • TPM3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • TPM3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • TPM3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene TPM3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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