IRAK4 (NM_016123) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC203041. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 51.3 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Protein KinaseDruggable Genome Protein Pathways: ApoptosisToll-like receptor signaling pathwayNeurotrophin signaling pathway

Protein Data

RefSeq: NP_057207	RefSeq Size: 2817	RefSeq ORF: 1383
LocusID: 51135	Cytogenetic: 12q12
Synonyms : IPD1; IRAK-4; NY-REN-64; REN64
Summary: This gene encodes a kinase that activates NF-kappaB in both the Toll-like receptor (TLR) and T-cell receptor (TCR) signaling pathways. The protein is essential for most innate immune responses. Mutations in this gene result in IRAK4 deficiency and recurrent invasive pneumococcal disease. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of interleukin-1 receptor-associated kinase 4 (IRAK4), transcript variant 2 Validated Antibodies • IRAK4 Rabbit anti-Mouse Polyclonal (aa38-54) Antibody • Rabbit monoclonal antibody against IRAK4, N-term(Y279) • Anti-TRAF6 Rabbit Polyclonal Antibody • Purified Goat Polyclonal Antibody against IRAK4 (N Terminus) • IRAK4 Rabbit Polyclonal (C-Terminus) Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • IRAK4 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • IRAK4 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Irak4 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • IRAK4 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Irak4 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene IRAK4

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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