CCL2 (NM_002982) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC202180. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 10.8 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinDruggable Genome Protein Pathways: Cytokine-cytokine receptor interactionChemokine signaling pathwayNOD-like receptor signaling pathway

Protein Data

RefSeq: NP_002973	RefSeq Size: 760	RefSeq ORF: 300
LocusID: 6347	Cytogenetic: 17q11.2
Synonyms : GDCF-2; HC11; HSMCR30; MCAF; MCP-1; MCP1; SCYA2; SMC-CF
Summary: This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens chemokine (C-C motif) ligand 2 (CCL2) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens chemokine (C-C motif) ligand 2 (CCL2) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens chemokine (C-C motif) ligand 2 (CCL2) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of chemokine (C-C motif) ligand 2 (CCL2) Validated Antibodies • ELISA and Luminex validated purified MCP-1 Mouse monoclonal antibody clone (5D3), capture • ELISA and Luminex validated purified biotinylated MCP-1 Mouse monoclonal antibody clone (2H5), detection • Rabbit Polyclonal Antibody against CCL2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CCL2 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CCL2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ccl12 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CCL2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ccl12 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CCL2

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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