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Home Recombinant Human Proteins All CHEK2 Proteins

CHEK2 (NM_007194) Purified Human Protein

Specifications Citations Customer Service Product Documents
Cat. No. Description Datasheet Price Availability*  
TP301278 Recombinant protein of human CHK2 checkpoint homolog (S. pombe) (CHEK2), transcript variant 1, 20 ug (Larger pack size?) 680
$398
In Stock
TA500398 CHEK2 (CHK2) mouse monoclonal antibody, clone 5C4 $379 In Stock
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OriGene Data
Species:Human Expression Host:HEK293 cells
Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC201278. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:C-terminal MYC/DDK Predicted MW:60.7 kDa
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:>50 ug/mL as determined by microplate BCA method
Buffer:25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
BioActivity: CHEK2 activity verified in a biochemical assay: CHEK2 (CHK2 checkpoint homolog) (TP301278) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. CHEK2 is a serine/threonine kinase that is involved in the control of the cell cycle. Varying concentrations of CHEK2 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
Protein Families: Protein KinaseStem cell - PluripotencyDruggable Genome
Protein Pathways: Cell cyclep53 signaling pathway
Protein Data
Protein Image
BioActivity Data
BioActivity Image
 

Reference Data
RefSeq: NP_009125 RefSeq Size: 1862 RefSeq ORF: 1632
LocusID: 11200 Cytogenetic: 22q12.1  
Synonyms : CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53
Summary:
*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information
**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

 

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