STIP1 (NM_006819) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201084. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 62.5 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Stem cell - Pluripotency Protein Pathways: Prion diseases

Protein Data

RefSeq: NP_006810	RefSeq Size: 2219	RefSeq ORF: 1632
LocusID: 10963	Cytogenetic: 11q13
Synonyms : HOP; IEF-SSP-3521; P60; STI1; STI1L
Summary: STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009].

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Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens stress-induced-phosphoprotein 1 (STIP1) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens stress-induced-phosphoprotein 1 (STIP1) as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of stress-induced-phosphoprotein 1 (STIP1) Validated Antibodies • Rabbit Polyclonal antibody to STIP1 (stress-induced-phosphoprotein 1) • Rabbit Monoclonal antibody against STIP1 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • STIP1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • STIP1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Stip1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • STIP1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Stip1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene STIP1

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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