STAT1 (NM_139266) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201075. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 82.9 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Transcription FactorsDruggable Genome Protein Pathways: Chemokine signaling pathwayToll-like receptor signaling pathwayJak-STAT signaling pathwayPathways in cancerPancreatic cancer

Protein Data

RefSeq: NP_644671	RefSeq Size: 2762	RefSeq ORF: 2139
LocusID: 6772	Cytogenetic: 2q32.2
Synonyms : CANDF7; ISGF-3; STAT91
Summary: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta as transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant beta Validated Antibodies • Purified STAT1 mouse monoclonal antibody, clone 9B12 • Purified STAT1 mouse monoclonal antibody, clone 4H9 • Purified anti-STAT1 mouse monoclonal antibody, clone 13B10 • Purified anti-STAT1 mouse monoclonal antibody, clone 15H3 • Rabbit monoclonal antibody against Stat-1 (N-term) (Y93) • Rabbit monoclonal antibody against Stat-1 (clone EPYR2154 ) • Rabbit monoclonal antibody against Stat-1 Phospho (pY701)(clone EPR3147) (Phospho-Specific) • Rabbit monoclonal antibody against Stat-1 Phospho (pS727)(clone EPR3146) (Phospho-Specific) • Rabbit polyclonal STAT1 (Ab-727) antibody • Rabbit polyclonal STAT1 (Ab-701) antibody • Rabbit polyclonal anti-STAT1 antibody • Rabbit polyclonal STAT1 (Tyr701) antibody(Phospho-specific) • Rabbit polyclonal STAT1 (Ser727) antibody(Phospho-specific) • Carrier-free (BSA/glycerol-free) STAT1 mouse monoclonal antibody, clone 9B12 • Carrier-free (BSA/glycerol-free) STAT1 mouse monoclonal antibody, clone 4H9 • Carrier-free (BSA/glycerol-free) STAT1 mouse monoclonal antibody, clone 13B10 • Carrier-free (BSA/glycerol-free) STAT1 mouse monoclonal antibody, clone 15H3 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • STAT1 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • STAT1 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Stat1 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • STAT1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Stat1 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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