CA3 (NM_005181) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC201066. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 29.4 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Druggable Genome Protein Pathways: Nitrogen metabolism

Protein Data

RefSeq: NP_005172	RefSeq Size: 2357	RefSeq ORF: 783
LocusID: 761	Cytogenetic: 8q21.2
Synonyms : CAIII; Car3
Summary: Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens carbonic anhydrase III, muscle specific (CA3) as transfection-ready DNA • Lenti ORF clone of Human carbonic anhydrase III, muscle specific (CA3) , Myc-DDK-tagged • Lenti ORF clone of Human carbonic anhydrase III, muscle specific (CA3), mGFP tagged • GFP-tagged ORF clone of Homo sapiens carbonic anhydrase III, muscle specific (CA3) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens carbonic anhydrase III, muscle specific (CA3) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of carbonic anhydrase III, muscle specific (CA3) Validated Antibodies • Rabbit polyclonal anti-CA3 antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • CA3 - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • CA3 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Car3 - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • CA3 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Car3 - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene CA3

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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