PPP2R5D (NM_180976) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200647. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 66 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Phosphatase Protein Pathways: Oocyte meiosisWnt signaling pathway

Protein Data

RefSeq: NP_851307	RefSeq Size: 2871	RefSeq ORF: 1713
LocusID: 5528	Cytogenetic: 6p21.1
Synonyms : B56D
Summary: The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 2 as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 2 as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens protein phosphatase 2, regulatory subunit B', delta (PPP2R5D), transcript variant 2 as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of protein phosphatase 2, regulatory subunit B', delta isoform (PPP2R5D), transcript variant 2 • Transient overexpression lysate of protein phosphatase 2, regulatory subunit B', delta isoform (PPP2R5D), transcript variant 2 Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • PPP2R5D - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • PPP2R5D - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Ppp2r5d - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • PPP2R5D - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Ppp2r5d - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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