||Expression Host:||HEK293 cells
|Expression cDNA Clone or AA Sequence
Recombinant protein was produced with TrueORF clone, RC200549
. Click on the TrueORF clone link to view cDNA and protein sequences.
||Predicted MW:||61.6 kDa|
|Purity:||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Concentration:||>50 ug/mL as determined by microplate BCA method|
|Buffer:||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.|
||Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
||RPS6KA5 activity verified in a biochemical assay: RPS6KA5 (ribosomal protein S6 kinase, 90kDa, polypeptide 5) (TP300549) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. RPS6KA5 is a serine/threonine kinase that is involved in the stress or mitogen-induced phosphorylation of various proteins including the transcription factors CREB, ATF1, and RELA, histone H2A and H3 and HMG-14. Varying concentrations of RPS6KA5 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
||Protein KinaseTranscription FactorsDruggable Genome
||MAPK signaling pathwayNeurotrophin signaling pathwayBladder cancer