| Species: | Human |
Expression Host: | Human 293HEK cells |
| Expression cDNA Clone and Sequence |
Recombinant protein was produced with TrueORF clone, RC200549. Click on the TrueORF clone link to view cDNA and protein sequences. |
| Tag: | C-terminal MYC/DDK |
Predicted MW: | 61.6 kDa |
| Purity: | > 80% as determined by SDS-PAGE and Coomassie blue staining |
| Mass Spec Validation: |
This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. |
| Concentration: | >50 ug/mL as determined by microplate BCA method |
| Buffer and Storage: | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. |
| Preparation: |
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. |
| BioActivity: |
RPS6KA5 activity verified in a biochemical assay: RPS6KA5 (ribosomal protein S6 kinase, 90kDa, polypeptide 5) (TP300549) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. RPS6KA5 is a serine/threonine kinase that is involved in the stress or mitogen-induced phosphorylation of various proteins including the transcription factors CREB, ATF1, and RELA, histone H2A and H3 and HMG-14. Varying concentrations of RPS6KA5 were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors. |
| Protein Families: |
Protein KinaseTranscription FactorsDruggable Genome |
| Protein Pathways: |
MAPK signaling pathwayNeurotrophin signaling pathwayBladder cancer |
