CDC25A (NM_001789) Purified Human Protein

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Cat. No.	Description	Datasheet	Price	Availability*
TP300496	Purified recombinant protein of Homo sapiens cell division cycle 25 homolog A (S. pombe) (CDC25A), transcript variant 1, 20 ug (Larger pack size?)		$680	In Stock
TA50011-100	4C5, Anti-DDK monoclonal antibody (100 μL)		$248	In Stock

Also for CDC25A (NM_001789)

OriGene Data

Species:	Human	Expression Host:	Human 293HEK cells
Expression cDNA Clone and Sequence	Recombinant protein was produced with TrueORF clone, RC200496. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag:	C-terminal MYC/DDK	Predicted MW:	58.9 kDa
Purity:	> 80% as determined by SDS-PAGE and Coomassie blue staining
Mass Spec Validation:	This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology.
Concentration:	>50 ug/mL as determined by microplate BCA method
Buffer and Storage:	25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
Preparation:	Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
Protein Families:	Phosphatase Druggable Genome
Protein Pathways:	Cell cycle Progesterone-mediated oocyte maturation

Protein Data

Reference Data

RefSeq: NP_001780	RefSeq Size: 3717	RefSeq ORF: 1575
LocusID: 993	Cytogenetic: 3p21
Synonyms : CDC25A2
Summary: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].

*: Inventory for certain proteins may be limited due to low expression level. Delivery time may vary from web posted schedule. Contact techsupport@origene.com for specific inventory information

**: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

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Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
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