APOE (NM_000041) Purified Human Protein

Species: Human Expression Host: Human 293HEK cells Expression cDNA Clone and Sequence Recombinant protein was produced with TrueORF clone, RC200395. Click on the TrueORF clone link to view cDNA and protein sequences. Tag: C-terminal MYC/DDK Predicted MW: 34.2 kDa Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining Mass Spec Validation: This protein has been positively validated by MS/MS by Dr. Robert Moritz at The Institute of Systems Biology. Concentration: >50 ug/mL as determined by microplate BCA method Buffer and Storage: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions. Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. Protein Families: Secreted ProteinStem cell - PluripotencyAdult stem cellsDruggable Genome Protein Pathways: Alzheimer's disease

Protein Data

RefSeq: NP_000032	RefSeq Size: 1223	RefSeq ORF: 954
LocusID: 348	Cytogenetic: 19q13.2
Synonyms : AD2; LDLCQ5; LPG
Summary: Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq, Jul 2008].

Researchers who bought this clone have also purchased:

Related ORF Clones • Myc-DDK-tagged ORF clone of Homo sapiens apolipoprotein E (APOE) as transfection-ready DNA • GFP-tagged ORF clone of Homo sapiens apolipoprotein E (APOE) as transfection-ready DNA • Myc-DDK-tagged ORF clone of Homo sapiens apolipoprotein E (APOE) as 200 ug transfection-ready DNA Related Lysate from OriGene • Transient overexpression lysate of apolipoprotein E (APOE) Validated Antibodies • Rabbit Polyclonal Antibody against APOE • Rabbit Monoclonal Antibody against APOE (Clone EP1374Y) • Rabbit Monoclonal Antibody against APOE (Clone EP1373Y) • Purified Rabbit Polyclonal Antibody against APOE (C-term) • Purified Goat Polyclonal Antibody against APOE • Mouse Monoclonal ApoE4 Antibody (4E4) • Apolipoprotein E / APOE Goat Polyclonal Antibody • Apolipoprotein E / APOE Goat Polyclonal (C-Terminus) Antibody • Anti-CXADR Mouse Monoclonal Antibody Anti-Tag Antibodies & WB Reagent • Anti-Tag Antibodies • Western Blot Reagent shRNA • APOE - Human, 4 unique 29mer shRNA constructs in retroviral RFP vector • APOE - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector • Apoe - Mouse, 4 unique 29mer shRNA constructs in retroviral GFP vector • APOE - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector • Apoe - Mouse, 4 unique 29mer shRNA constructs in retroviral untagged vector Related Primer Pair qSTAR qPCR primer pairs against Homo sapiens gene APOE

If you would like OriGene Technologies to prepare a purified protein in the quantity of your own interest, simply fill out the form and click Submit. OriGene will contact you.

Protein	Full Length Protein A domain of the protein Description of domain...
Tag	C-fusion N-fusion     Tag Description:
DNA or protein sequence of the final product
Preferred Expression Host	HEK293 E. coli Insect Cells Yeast
The main application with the purified protein	R&D reagent Cell-based Assay Animal Study Other, please specify:
* Quantity (µg)
Comments
* First Name
* Last Name
* Email Address
* Institution Name
* Country
* Phone