BC100379 Mouse Tagged ORF Clone
CAT#: MG201192
- TrueORF®
(tGFP-tagged) - Mouse cDNA clone MGC:117858 IMAGE:30460254
ORF Plasmid: DDK
"BC100379" in other vectors (3)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201192 representing BC100379
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTATGGGTCGGCAGGACCGCAGGCTGCAGCAGCAGCTTAATGAAAGCAGGAGCCGCTTCCAGA CGTTAATGAAGCGGCTGATAGCGAAGTACAACCAGCCCTTTAAGGATAACTATGATGAGCTTAAAAAAGA ATTCAACAGACTTTATCAGAAGTATTGTCTGTCACCGCAGCGGGCGAAGGTGACTTCGTGTGGCAGAGTA TCGCCAATGAAAGCTGCTGCAGCTCTTCCCTGTCAATCAGAGCACTTGAAGAGACTAAATCCAGACTCCC CACAACAGAGTTCCCAGAAACGGTCAATCTCTCCTGGCTGTCACAGAAGAGTCCTGCAGGATTCAACTCC ACAGACTGCTAGTACACTTGTGAGAGAGCCTTGGCTTCCAACAAAGAGGCGCAAGTTGTCATACCCGGTA GCTTGTGCTCACCAGGCCAAATCTCACAATACCTCAGGAGCATCAGGCTGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201192 representing BC100379
Red=Cloning site Green=Tags(s) MESMGRQDRRLQQQLNESRSRFQTLMKRLIAKYNQPFKDNYDELKKEFNRLYQKYCLSPQRAKVTSCGRV SPMKAAAALPCQSEHLKRLNPDSPQQSSQKRSISPGCHRRVLQDSTPQTASTLVREPWLPTKRRKLSYPV ACAHQAKSHNTSGASGWP TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC100379 |
ORF Size | 474 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC100379.1 |
RefSeq Size | 2008 bp |
RefSeq ORF | 476 bp |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR201192 | (Myc-DDK-tagged) - Mouse cDNA clone MGC:117858 IMAGE:30460254 |
USD 165.00 |
|
MR201192L3 | Lenti ORF clone of MGC:11785 (Myc-DDK-tagged) - Mouse cDNA clone MGC:117858 IMAGE:30460254 |
USD 465.00 |
|
MR201192L4 | Lenti ORF clone of MGC:11785 (mGFP-tagged) - Mouse cDNA clone MGC:117858 IMAGE:30460254 |
USD 465.00 |
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