Pcdhb9 (NM_053134) Mouse Tagged ORF Clone

CAT#: MR210685

Pcdhb9 (Myc-DDK-tagged) - Mouse protocadherin beta 9 (Pcdhb9)

Product Images

Circular map for MR210685

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Pcdhb9
• Synonyms: Pcdhb4C; PcdhbI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR210685 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAAGCTGGGGAGAATTCACCTAAACAGGCAAGTAATGGCATTTATTTTTATGATGGTTTTGGTTC
  AGGTTTGCAGTGAGCCAACAATTCGATACTCTATCTTAGAGGAAACAGAAAGTGGCTCCTTTGTAGCCCA
  CCTGGCCAAGGATCTGGGTCTGGGAGCTAGGGAGCTGGCTGCCAGGTCTGCACGGGTGTTGTCTGATGAT
  TACAAGCAGAGATTACTGCTGGATCCTGAGACTGGAGATTTGCTTCTGAGGGAGAAAGTAGACCGGGAGG
  AGGTGTGTAGCACTGTGGATCCCTGTGTGCTGCATTTCCAGGTGACTCTTGAAAAGCCAGTGCAATATTT
  TCAAAGAGAATTATTGATCCAGGACATAAATGATCATGCTCCAGAATTCCCAGATAGAGAACTGCTCTTG
  AGAATACCAGAGAACAGCCAGCAAGGCACACAATTTTCACTGAATTTAGCCCAGGACTTGGATGTGGGCA
  GCAATGGGCTTCAACAATACACAGTCAGCCCCAACCCTTATTTTCACGTCCTCACTCAAAATAATAGTAA
  AGGCAAAAAATACCCAGAGTTGGTGCAGGACAGAGGCCTGGACAGAGAGGAGCAGGCAGAGCTGAGCTTA
  ACCCTCACAGCTCTGGATGGCGGGTCTCCACCGAGGTCAGGAACAGCCCTGGTTCGAATCCTGATCATGG
  ATATCAATGACAATGCTCCTGAGTTTGTGAACAGTCCCTATGAGGTGCAGGTCCTAGAGAGCAGTCCTCC
  AGACTCCCCAGTCTTGACTGTCTTAGCACGGGATGCAGATGCTGGCAACTTTGGGAGAGTTTCCTATGGC
  TTCTTTCAAGCATCAGATGAAATCCAACAAACATTCTCAATAAACGCAACTAGTGGAGACATGCGATTGA
  AAAAGAAATTGGATTTTGAAAAAATTAAATCTTACCATGTGGAAATCGAGGCCATAGATGGAGGTGGGCT
  TTCTGGGAAAGGCTCTGTAACGATAGAGGTGGTGGATGTGAATGACAATGCCCCAGAGCTGACCATATCT
  TCACTGACCAGCTCTGTCCCAGAAAATGCTCCTGAGACGATCATAAGCATCTTCAGAGTTGGGGACAGGG
  ATTCCGGAGAGAATGGAAAGATGGTTTGTTCTATTCCAGAGAATCTGCCATTTATTCTGAAATCCACCTT
  CAAGAACTTCTACACCCTGGTGACAGAGAGCCCTCTGGACAGAGAAAGCAGAGCTGAGTACAATATCACC
  ATCATGGTCTCTGACATGGGCACACCCAGGCTCACAACATGGCACACCATAAAGGTGCAGGTGTCCGACA
  TCAACGACAACACCCCCGCCTTCACCCAAACCTCCTACACCATGTTTGTCCGCGAGAACAACAGCCCTGC
  CCTGCACATAGGCACCATCAGTGCCACAGACTCAGACTCAGGCTCCAATGCCCACATCACCTACTCGCTG
  CTGCCGCCCCATGATCCGGAACTGGCCCTCTCTTCGTTAATCTCGATTAACGCTGACAATGGGCAGCTGT
  TTGCACTCAGGGCGCTGGACTATGAGGCCCTGCAGGTCTTCGAGTTCCACGTGGGCGCCACAGATGGAGG
  CTCACCTCCACTTAGCAGCCAGGCTCTGGTGCGCGTGGTGGTGCTGGATGACAATGACAATGCGCCCTTC
  GTGCTCTACCCTATGCAGAACGCCTCGGCTCCCTTCACTGAGCTGCTGCCTAGAGCTGCGGAGCCAGGCT
  ACCTGGTCACCAAGGTGGTTGCAGTGGACCGCGACTCTGGCCAGAATGCCTGGCTATCCTTCCAGTTGCT
  CAAGGCCACGGAACCTGGGCTGTTCAGCGTGTGGGCGCACAATGGAGAGGTGCGCACCACCAGGTTGTTG
  AGTGAGCGCGATGTGCCCAAGCACAGGCTACTGCTGGTGGTCAAGGACAATGGAGAGCCTCAGCGCTCTG
  CCAGTGTCACTCTGCAGGTGCTGTTAGTGGATGGCTTCTCACAATCTTACTTACCTCTTCCAGAGGTAGC
  GCGGGATCCCGCCCATGAAGATGAGGACGTGCTTACATTGTACCTGGTCATTGCCTTGGCATCTGTGTCT
  TCTCTCTTCCTCCTGTCTGTGCTGCTGTTTGTGGGGGTAAGACTGTGCAGAAGGGCCAGAGAGGTCTCTC
  TGGGTGGCTGCTCTATGCCTGGTGAACACTTTCCTGGCCACCTGGTCGATGTCAGTGGAGCAGGGACCCT
  GTCCCAAAGCTACCAGTATGAGGTGTGTCTGAGGGGCGACTCTGGGACTGGTGAGTTCAAGTTCCTGAAA
  CCCATGATCCCCAATGCTGGGATAGAAATTATGGAAAGTCCCCACTGCAGGGATAGCTTTGTATTCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR210685 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKLGRIHLNRQVMAFIFMMVLVQVCSEPTIRYSILEETESGSFVAHLAKDLGLGARELAARSARVLSDD
  YKQRLLLDPETGDLLLREKVDREEVCSTVDPCVLHFQVTLEKPVQYFQRELLIQDINDHAPEFPDRELLL
  RIPENSQQGTQFSLNLAQDLDVGSNGLQQYTVSPNPYFHVLTQNNSKGKKYPELVQDRGLDREEQAELSL
  TLTALDGGSPPRSGTALVRILIMDINDNAPEFVNSPYEVQVLESSPPDSPVLTVLARDADAGNFGRVSYG
  FFQASDEIQQTFSINATSGDMRLKKKLDFEKIKSYHVEIEAIDGGGLSGKGSVTIEVVDVNDNAPELTIS
  SLTSSVPENAPETIISIFRVGDRDSGENGKMVCSIPENLPFILKSTFKNFYTLVTESPLDRESRAEYNIT
  IMVSDMGTPRLTTWHTIKVQVSDINDNTPAFTQTSYTMFVRENNSPALHIGTISATDSDSGSNAHITYSL
  LPPHDPELALSSLISINADNGQLFALRALDYEALQVFEFHVGATDGGSPPLSSQALVRVVVLDDNDNAPF
  VLYPMQNASAPFTELLPRAAEPGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVRTTRLL
  SERDVPKHRLLLVVKDNGEPQRSASVTLQVLLVDGFSQSYLPLPEVARDPAHEDEDVLTLYLVIALASVS
  SLFLLSVLLFVGVRLCRRAREVSLGGCSMPGEHFPGHLVDVSGAGTLSQSYQYEVCLRGDSGTGEFKFLK
  PMIPNAGIEIMESPHCRDSFVFN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053134
• ORF Size: 2382 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053134.3, NP_444364.3
• RefSeq Size: 3055 bp
• RefSeq ORF: 2487 bp
• Locus ID: 93880
• Cytogenetics: 18 B3
• MW: 87.4 kDa