F11r (NM_172647) Mouse Tagged ORF Clone

CAT#: MR204187

F11r (Myc-DDK-tagged) - Mouse F11 receptor (F11r)

Product Images

Circular map for MR204187

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: F11r
• Synonyms: 9130004G24; AA638916; ESTM33; JAM; JAM-1; JAM-A; Jcam; Jcam1; Ly106
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR204187 representing NM_172647
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCACCGAGGGGAAAGCCGGGAGGAAACTGTTGTTTCTCTTCACGTCTATGATCCTGGGCTCTTTGG
  TACAAGGCAAGGGTTCGGTGTACACTGCTCAATCTGACGTCCAGGTTCCCGAGAACGAGTCCATCAAATT
  GACCTGCACCTACTCTGGCTTCTCCTCTCCCCGAGTGGAGTGGAAGTTCGTCCAAGGCAGCACAACTGCA
  CTTGTGTGTTATAACAGCCAGATCACAGCTCCCTATGCGG[AT]CCGAGTCACCTTCTCATCCAGTGGCA
  TCACGTTCAGTTCTGTGACCCGGAAGGACAATGGAGAGTATACTTGCATGGTCTCCGAGGAAGGTGGCCA
  GAACTACGGGGAGGTCAGCATCCACCTCACTGTGCTTGTACCTCCATCCAAGCCGACGATCAGTGTCCCC
  TCCTCTGTCACCATTGGGAACAGGGCAGTGCTGACCTGCTCAGAGCATGATGGTTCCCCACCCTCTGAAT
  ATTCCTGGTTCAAGGACGGGATATCCATGCTTACAGCAGATGCCAAGAAAACCCGGGCCTTCATGAATTC
  TTCATTCACTATTGATCCAAAGTCGGGGGATCTGATCTTTGACCCCGTGACAGCCTTTGATAGTGGTGAA
  TACTACTGCCAGGCCCAGAATGGATATGGGACAGCCATGAGGTCAGAGGCTGCACACATGGATGCTGTGG
  AGCTGAATGTGGGGGGCATCGTGGCAGCTGTCCTGGTAACACTGATTCTCCTTGGACTCTTGATTTTTGG
  CGTCTGGTTTGCCTATAGCCGTGGATACTTTGAAAGAACAAAGAAAGGGACTGCACCGGGTAAGAAGGTC
  ATTTACAGCCAGCCCAGTACTCGAAGTGAGGGGGAATTCAAACAGACCTCGTCGTTCCTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR204187 representing NM_172647
  Red=Cloning site Green=Tags(s)
  
  MGTEGKAGRKLLFLFTSMILGSLVQGKGSVYTAQSDVQVPENESIKLTCTYSGFSSPRVEWKFVQGSTTA
  LVCYNSQITAPYAXXRVTFSSSGITFSSVTRKDNGEYTCMVSEEGGQNYGEVSIHLTVLVPPSKPTISVP
  SSVTIGNRAVLTCSEHDGSPPSEYSWFKDGISMLTADAKKTRAFMNSSFTIDPKSGDLIFDPVTAFDSGE
  YYCQAQNGYGTAMRSEAAHMDAVELNVGGIVAAVLVTLILLGLLIFGVWFAYSRGYFERTKKGTAPGKKV
  IYSQPSTRSEGEFKQTSSFLV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172647
• ORF Size: 903 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172647.1, NM_172647.2, NP_766235.1
• RefSeq Size: 2482 bp
• RefSeq ORF: 903 bp
• Locus ID: 16456
• UniProt ID: O88792
• Cytogenetics: 1 79.43 cM
• MW: 32.9 kDa
• Gene Summary: Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3 (PubMed:11447115). The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (PubMed:11447115). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier (PubMed:9660867). Ligand for integrin alpha-L/beta-2 involved in memory T-cell and neutrophil transmigration (By similarity). Involved in platelet activation (By similarity).[UniProtKB/Swiss-Prot Function]