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Home Recombinant Human Proteins DAP3 Heavy-labeled protein

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DAP3 Mass Spec Standard

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Cat. No. Ref. ID Description Price Availability
PH323182 NM_033657 DAP3 MS Standard C13 and N15-labeled recombinant protein (NP_387506), 10 ug $1500 3 weeks
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Also for DAP3 (NM_033657)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data
Expression Host:Human HEK293 cells Predicted MW:45.4 kDa
Expression TrueORF clone:RC223182 Protein Sequence: Sequence Link
Tag:C-terminal MYC/DDK * Anti-Myc/DDK antibody Protein family:
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:> 50 ug/ml as determined by BCA
Labeling Method:Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer and Storage:100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Protein Image
*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

Reference Data
RefSeq: NP_387506 RefSeq Size: 1650 RefSeq ORF: 1197
Synonyms : bMRP-10; DAP-3; MRP-S29; MRPS29
LocusID: 7818 Cytogenetic: 1q22  
Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq, Dec 2010].

 

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