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Home Recombinant Human Proteins F10 Heavy-labeled protein

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F10 Mass Spec Standard

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Cat. No. Ref. ID Description Price Availability
PH308506 NM_000504 F10 MS Standard C13 and N15-labeled recombinant protein (NP_000495), 10 ug $1500 3 weeks
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Also for F10 (NM_000504)
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OriGene Data
Expression Host:Human HEK293 cells Predicted MW:52.3 kDa
Expression TrueORF clone:RC208506 Protein Sequence: Sequence Link
Tag:C-terminal MYC/DDK * Anti-Myc/DDK antibody Protein family:
Purity:> 80% as determined by SDS-PAGE and Coomassie blue staining
Concentration:> 50 ug/ml as determined by BCA
Labeling Method:Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
Buffer and Storage:100 mM glycine, 25 mM Tris-HCl, pH 7.3. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Protein Image
*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich

Reference Data
RefSeq: NP_000495 RefSeq Size: 1560 RefSeq ORF: 1467
Synonyms : FX; FXA
LocusID: 2159 Cytogenetic: 13q34  
Summary: This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. [provided by RefSeq, Jul 2008].

 

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