Home Over-expression Lysates AKR1C4 Lysate|
AKR1C4 Over-expression Lysate Product
- Transient overexpression lysate of aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4) (AKR1C4) , 100ug (for larger package size, click here)
- LY40001, Empty vector transfected control cell lysate (HEK293 cell lysate), 100ug. Included for Free
Also for AKR1C4 (NM_001818)
|Expression cell line:
||Human HEK293T cell line
|Expression cDNA Clone:
||TrueORF Clone RC204731|
|Epitope tag:||C-terminal Myc/DDK*|
|Detection Antibodies:||Anti-DDK Antibody (TA50011)|
||Western Blot experiments.
Left-Control; Right -Over-expression Lysate
|Accession Number:||NM_001818 NP_001809|
|Other Names:||AKR1C4,3-alpha-HSD; C11; CDR; CHDR; DD-4; DD4; HAKRA
|Predicted MW:||36.9 kDa|
|Components:||1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer|
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 0.5 ml 2xSDS Sample Buffer (4%SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 5% b-mercaptoethanol)
|Storage:||The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles. |
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
|Preparation:||HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20ºC before shipping.
||Primary bile acid biosynthesisC21-Steroid hormone metabolismAndrogen and estrogen metabolismMetabolism of xenobiotics by cytochrome P450Metabolic pathways
*: DDK-tag is the same as FLAG tag. Flag® is a registered trademark of Sigma-Aldrich