ACAT1 (ACACA) (NM_198834) Human Untagged Clone

CAT#: SC307787

ACACA (untagged)-Human acetyl-CoA carboxylase alpha (ACACA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACAT1
• Synonyms: ACAC; ACACAD; ACC; ACC1; ACCA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_198834 edited
  ATGTGGTGGTCTACTCTGATGTCAATCTTGAGGGCTAGGTCTTTCTGGAAGTGGATATCT
  ACTCAGACAGTAAGAATTATAAGAGCTGTAAGAGCTCATTTTGGAGGAATAATGGATGAA
  CCATCTCCCTTGGCCCAACCTCTGGAGCTGAACCAGCACTCTCGATTCATAATAGGTTCT
  GTGTCTGAAGATAACTCAGAGGATGAGATCAGCAACCTGGTGAAGTTGGACCTACTGGAG
  GAGAAGGAGGGCTCCTTGTCACCTGCTTCTGTTGGCTCAGATACACTCTCTGATTTGGGG
  ATCTCTAGCCTACAGGATGGCTTGGCCTTGCACATAAGGTCCAGCATGTCTGGCTTGCAC
  CTAGTAAAGCAGGGCCGAGACAGAAAGAAAATAGATTCTCAACGAGATTTCACTGTGGCT
  TCTCCAGCAGAATTTGTTACTCGCTTTGGGGGAAATAAAGTGATTGAGAAGGTTCTTATT
  GCTAACAATGGCATTGCAGCAGTGAAATGCATGCGGTCTATCCGTAGGTGGTCTTATGAA
  ATGTTTCGAAATGAACGTGCAATTAGATTCGTTGTCATGGTCACACCTGAAGACCTTAAA
  GCCAATGCAGAATACATTAAGATGGCAGATCACTATGTGCCAGTGCCTGGAGGACCAAAC
  AACAACAACTATGCAAATGTGGAATTAATTCTTGATATTGCTAAAAGGATCCCAGTACAA
  GCAGTGTGGGCTGGCTGGGGTCATGCTTCTGAGAATCCCAAACTACCGGAACTTCTCTTG
  AAAAATGGCATTGCCTTCATGGGTCCTCCAAGCCAGGCCATGTGGGCTTTAGGGGATAAG
  ATTGCATCTTCCATAGTGGCTCAAACTGCAGGTATCCCAACTCTTCCCTGGAGCGGCAGT
  GGTCTTCGTGTGGACTGGCAGGAAAATGATTTTTCAAAACGTATCTTAAATGTTCCCCAG
  GAGCTATATGAAAAAGGTTATGTGAAAGATGTGGATGATGGGCTACAGGCAGCTGAGGAA
  GTTGGATATCCAGTAATGATCAAGGCCTCAGAGGGAGGAGGAGGGAAGGGAATTAGAAAA
  GTCAACAATGCAGATGACTTCCCTAATCTCTTCAGACAGGTTCAAGCTGAAGTTCCTGGA
  TCTCCCATATTTGTGATGAGACTAGCCAAACAATCTCGTCATCTGGAGGTGCAGATCTTA
  GCGGACCAATATGGCAATGCTATCTCTTTGTTTGGTCGTGATTGCTCTGTACAACGCAGG
  CATCAGAAGATTATTGAAGAAGCACCTGCTACTATTGCTACTCCAGCAGTATTTGAACAC
  ATGGAACAGTGTGCGGTGAAACTTGCCAAAATGGTGGGTTATGTGAGTGCTGGGACTGTG
  GAATACCTGTACAGCCAGGATGGCAGCTTCTACTTTCTGGAATTGAATCCTCGGCTGCAG
  GTAGAGCACCCTTGTACAGAGATGGTGGCTGATGTCAATCTCCCTGCAGCACAGCTCCAG
  ATTGCCATGGGGATTCCTCTATATAGAATCAAGGATATCCGTATGATGTATGGGGTATCT
  CCCTGGGGTGATTCTCCCATTGATTTTGAAGATTCTGCACACGTTCCTTGTCCAAGGGGC
  CATGTTATTGCTGCTCGGATCACTAGTGAAAATCCAGATGAGGGTTTTAAGCCCAGCTCA
  GGAACAGTTCAGGAGCTAAATTTCCGCAGCAATAAGAATGTTTGGGGATATTTCAGTGTT
  GCTGCTGCAGGGGGACTTCATGAATTTGCTGATTCTCAGTTTGGTCACTGCTTTTCTTGG
  GGAGAAAACAGAGAAGAGGCAATTTCAAACATGGTGGTGGCTTTGAAGGAGCTGTCTATT
  CGGGGTGACTTTCGAACTACAGTTGAATACCTGATCAAATTGTTAGAGACTGAAAGCTTT
  CAGATGAACAGAATTGATACTGGCTGGCTGGACAGACTGATAGCAGAAAAAGTACAGGCT
  GAGCGACCTGACACCATGTTGGGGGTTGTGTGTGGTGCCCTCCACGTGGCAGATGTGAGC
  CTGCGGAATAGCGTCTCTAACTTCCTTCACTCCTTAGAAAGGGGTCAAGTCCTTCCTGCT
  CATACACTTCTGAATACAGTAGATGTTGAACTTATCTATGAGGGAGTCAAGTATGTACTT
  AAGGTGACTCGACAGTCCCCCAACTCCTATGTGGTGATCATGAATGGCTCATGTGTAGAA
  GTAGATGTACATCGGCTGAGTGACGGTGGACTGCTCTTGTCCTATGATGGCAGCAGTTAT
  ACTACGTATATGAAAGAGGAAGTGGATAGATATCGCATCACAATTGGCAATAAAACCTGT
  GTGTTTGAGAAGGAAAATGACCCATCGGTGATGCGCTCACCTTCTGCTGGGAAGTTAATC
  CAGTACATTGTAGAAGATGGAGGTCATGTGTTTGCCGGCCAGTGCTATGCTGAGATTGAG
  GTAATGAAGATGGTAATGACCTTAACAGCTGTGGAGTCTGGCTGTATCCATTACGTCAAG
  CGACCTGGAGCAGCTCTTGACCCTGGCTGTGTACTAGCCAAAATGCAACTGGACAACCCC
  AGCAAGGTTCAGCAGGCTGAACTTCACACAGGTAGTCTGCCACGGATCCAGAGCACGGCA
  CTCAGAGGCGAGAAACTCCATCGAGTGTTCCATTATGTCCTGGATAATCTGGTCAATGTA
  ATGAATGGATACTGCCTTCCAGATCCTTTCTTTAGCAGCAAGGTAAAAGACTGGGTAGAG
  CGATTGATGAAAACCCTCAGAGATCCCTCCCTGCCTCTCCTAGAATTGCAAGATATTATG
  ACCAGTGTGTCTGGCCGCATTCCCCCCAATGTGGAGAAGTCTATCAAGAAGGAAATGGCT
  CAGTATGCTAGCAACATCACATCAGTCCTCTGTCAGTTTCCCAGCCAGCAGATTGCAAAC
  ATCCTAGATAGCCATGCAGCTACATTGAACCGGAAATCTGAACGGGAAGTCTTCTTTATG
  AATACTCAGAGCATTGTTCAGCTGGTACAGAGGTACCGAAGTGGCATCCGAGGCCACATG
  AAGGCTGTGGTGATGGATCTGCTCCGGCAGTACCTGCGAGTAGAGACACAATTCCAGAAT
  GGTCACTATGACAAATGTGTATTCGCCCTCCGAGAAGAGAATAAAAGTGACATGAACACT
  GTACTGAACTACATCTTCTCTCACGCTCAAGTCACCAAGAAGAATCTTCTGGTCACAATG
  CTTATTGATCAGTTGTGTGGCCGGGACCCTACTCTCACTGATGAGCTGCTGAATATTCTC
  ACAGAGCTAACTCAACTCAGTAAGACCACCAATGCCAAAGTAGCACTTCGAGCACGCCAG
  GTTCTTATTGCCTCCCATTTGCCATCATATGAGCTTCGCCATAACCAAGTAGAGTCTATC
  TTCCTATCAGCTATTGACATGTATGGACATCAATTTTGCATTGAGAACCTGCAGAAACTC
  ATCCTATCAGAAACATCTATTTTTGATGTCCTACCAAACTTCTTCTATCACAGCAACCAA
  GTAGTGAGGATGGCAGCTCTGGAGGTGTATGTTCGAAGGGCTTATATTGCCTATGAACTT
  AACAGCGTACAACACCGCCAGCTTAAGGACAACACCTGTGTGGTGGAATTCCAGTTCATG
  CTGCCCACATCTCATCCAAACAGAGGGAACATCCCTACGCTAAACAGAATGTCCTTCTCC
  TCCAACCTCAACCACTATGGCATGACCCATGTAGCTAGTGTCAGCGATGTACTGTTGGAC
  AACTCATTCACTCCACCTTGTCAGCGGATGGGCGGAATGGTCTCTTTTCGGACTTTTGAA
  GATTTTGTCAGGATCTTTGATGAAGTGATGGGCTGCTTCTCTGACTCCCCACCCCAGAGT
  CCCACATTCCCTGAGGCAGGTCACACGTCTCTTTATGATGAGGATAAGGTTCCCAGGGAT
  GAACCAATTCACATTCTCAATGTGGCTATCAAGACTGACTGTGATATTGAGGATGACAGG
  CTGGCAGCTATGTTCAGAGAATTTACCCAGCAAAATAAAGCTACCCTGGTTGACCATGGG
  ATCCGGCGCCTTACTTTCCTGGTTGCACAAAAGGATTTCAGAAAGCAGGTCAACTATGAG
  GTGGATCGGAGATTTCATAGAGAATTCCCTAAATTTTTTACATTCCGAGCAAGGGATAAG
  TTTGAGGAGGATCGTATCTATCGTCATCTGGAGCCTGCTCTGGCTTTCCAGTTAGAGCTG
  AACCGGATGAGAAATTTTGACCTCACTGCCATTCCATGTGCTAATCACAAGATGCACCTG
  TATCTCGGGGCAGCCAAGGTGGAAGTGGGCACAGAAGTGACAGACTACAGGTTCTTTGTT
  CGTGCAATCATCAGGCATTCTGATCTGGTCACCAAGGAAGCTTCTTTTGAATATCTGCAA
  AATGAAGGGGAGCGGCTACTCCTGGAAGCCATGGATGAGTTGGAAGTTGCTTTTAACAAT
  ACAAATGTCCGCACTGACTGTAACCACATCTTCCTCAACTTTGTGCCCACGGTTATCATG
  GACCCATCAAAGATTGAGGAATCCGTGCGGAGCATGGTAATGCGGTATGGAAGTCGCCTG
  TGGAAATTGCGCGTCCTCCAGGCAGAACTGAAAATCAACATTCGCCTGACGCCAACTGGA
  AAAGCAATTCCCATCCGCCTCTTCCTGACAAACGAGTCTGGCTATTACTTGGATATCAGC
  CTATACAAGGAAGTGACTGACTCCAGGACAGCACAGATCATGTTTCAGGCATATGGAGAC
  AAACAGGGACCACTGCATGGAATGTTAATCAATACTCCATATGTGACCAAAGACCTGCTG
  CAATCAAAGAGGTTCCAGGCACAATCCTTAGGGACAACATACATATATGATATCCCAGAG
  ATGTTTCGGCAGTCCCTGATCAAACTCTGGGAGTCTATGTCCACTCAAGCATTTCTTCCA
  TCTCCCCCTCTGCCTTCTGACATGCTGACTTACACTGAACTGGTACTGGATGATCAAGGT
  CAGCTGGTCCACATGAACAGGCTTCCAGGAGGAAATGAGATTGGCATGGTAGCTTGGAAA
  ATGACCTTTAAAAGTCCTGAATATCCAGAAGGCCGAGATATCATTGTTATTGGCAATGAC
  ATCACATACCGAATTGGGTCCTTTGGGCCTCAAGAGGATTTGTTATTTCTCAGAGCTTCC
  GAACTTGCTAGGGCAGAAGGTATTCCACGCATCTATGTATCAGCCAACAGTGGAGCAAGA
  ATCGGACTGGCAGAAGAAATTCGCCATATGTTTCATGTGGCCTGGGTAGATCCTGAGGAT
  CCTTACAAGGGATACAGGTATTTATATCTGACTCCTCAAGATTATAAGAGAGTCAGTGCT
  CTCAACTCTGTCCATTGTGAACACGTGGAAGATGAAGGAGAATCCAGGTACAAGATAACA
  GATATTATTGGGAAAGAAGAGGGAATTGGACCCGAGAACCTTCGAGGTTCTGGAATGATT
  GCTGGAGAATCCTCATTGGCCTATAATGAGATCATTACCATCAGCCTGGTGACGTGCCGG
  GCCATTGGGATTGGGGCTTACCTTGTCCGGCTGGGACAGAGAACCATCCAGGTTGAGAAT
  TCTCACTTAATTCTAACAGGAGCTGGAGCCCTCAACAAAGTCCTCGGGCGGGAAGTGTAC
  ACCTCCAATAACCAGCTGGGGGGCATCCAGATTATGCACAACAATGGGGTGACCCACTGC
  ACTGTGTGTGATGACTTTGAAGGGGTTTTCACTGTCCTGCACTGGCTGTCTTACATGCCC
  AAGAGCGTGCACAGTTCAGTTCCTCTTCTGAACTCAAAGGATCCTATAGACAGAATCATC
  GAGTTTGTTCCCACAAAGACCCCATACGATCCTCGATGGATGCTAGCAGGCCGTCCTCAC
  CCAACCCAAAAAGGTCAGTGGTTGAGTGGCTTTTTTGACTATGGATCTTTCTCAGAGATT
  ATGCAGCCCTGGGCACAGACTGTGGTGGTTGGTAGAGCCAGGCTAGGAGGAATACCTGTG
  GGAGTTGTTGCTGTAGAAACCCGAACAGTAGAACTAAGTATCCCAGCTGATCCAGCAAAC
  CTGGATTCTGAAGCCAAGATAATCCAGCAGGCTGGCCAGGTTTGGTTCCCAGATTCTGCG
  TTTAAGACGTATCAGGCCATCAAGGACTTCAACCGGGAAGGGCTGCCTCTGATGGTCTTT
  GCCAACTGGAGAGGCTTCTCTGGTGGAATGAAAGATATGTACGACCAAGTGCTGAAGTTT
  GGTGCTTACATTGTGGATGGCTTGAGGGAGTGCTGCCAGCCTGTGCTGGTTTACATTCCT
  CCCCAGGCTGAGCTGCGGGGTGGCTCCTGGGTGGTGATTGACTCCTCCATCAACCCCCGG
  CACATGGAGATGTATGCTGACCGAGAAAGCAGGGGATCTGTTCTGGAGCCAGAAGGGACA
  GTAGAAATCAAATTCCGCAGAAAGGATCTGGTGAAAACCATGCGTCGGGTGGACCCAGTC
  TACATCCACTTGGCTGAGCGATTGGGGACCCCAGAGCTAAGCACAGCTGAGCGGAAGGAG
  TTGGAGAACAAGTTGAAGGAGCGGGAGGAATTCCTAATTCCCATTTACCATCAGGTAGCC
  GTGCAGTTTGCTGACTTGCACGACACACCAGGCCGGATGCAGGAGAAGGGTGTTATTAGC
  GATATCCTGGATTGGAAAACATCCCGTACCTTCTTCTACTGGCGGCTGAGGCGTCTTCTG
  CTGGAGGACCTGGTCAAGAAGAAAATCCACAATGCCAACCCTGAGCTGACTGATGGCCAG
  ATTCAAGCCATGTTAAGGCGCTGGTTTGTGGAAGTGGAAGGAACAGTGAAGGCTTATGTT
  TGGGACAATAATAAGGATCTGGCGGAGTGGCTAGAGAAACAGCTGACAGAGGAGGATGGT
  GTTCACTCGGTAATAGAGGAAAACATCAAATGCATCAGCAGAGACTACGTCCTCAAGCAA
  ATCCGCAGCTTGGTCCAGGCCAATCCAGAGGTTGCCATGGATTCCATCATCCATATGACG
  CAGCACATATCACCCACTCAGCGAGCAGAAGTCATACGGATCCTCTCCACAATGGATTCC
  CCTTCCACGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_198834
• Insert Size: 8000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: ORF of this clone was fully sequenced and it matches with NM_198834.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198834.1, NP_942131.1
• RefSeq Size: 10018 bp
• RefSeq ORF: 7152 bp
• Locus ID: 31
• UniProt ID: Q13085
• Cytogenetics: 17q12
• Protein Families: Druggable Genome
• Protein Pathways: Fatty acid biosynthesis, Insulin signaling pathway, Metabolic pathways, Propanoate metabolism, Pyruvate metabolism
• Gene Summary: Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (1).