TSH Receptor (TSHR) (NM_000369) Human Untagged Clone

CAT#: SC119936

TSHR (untagged)-Human thyroid stimulating hormone receptor (TSHR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TSH Receptor
• Synonyms: CHNG1; hTSHR-I; LGR3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119936 sequence for NM_000369 edited (data generated by NextGen Sequencing)
  ATGAGGCCGGCGGACTTGCTGCAGCTGGTGCTGCTGCTCGACCTGCCCAGGGACCTGGGC
  GGAATGGGGTGTTCGTCTCCACCCTGCGAGTGCCATCAGGAGGAGGACTTCAGAGTCACC
  TGCAAGGATATTCAACGCATCCCCAGCTTACCGCCCAGTACGCAGACTCTGAAGCTTATT
  GAGACTCACCTGAGAACTATTCCAAGTCATGCATTTTCTAATCTGCCCAATATTTCCAGA
  ATCTACGTATCTATAGATGTGACTCTGCAGCAGCTGGAATCACACTCCTTCTACAATTTG
  AGTAAAGTGACTCACATAGAAATTCGGAATACCAGGAACTTAACTTACATAGACCCTGAT
  GCCCTCAAAGAGCTCCCCCTCCTAAAGTTCCTTGGCATTTTCAACACTGGACTTAAAATG
  TTCCCTGACCTGACCAAAGTTTATTCCACTGATATATTCTTTATACTTGAAATTACAGAC
  AACCCTTACATGACGTCAATCCCTGTGAATGCTTTTCAGGGACTATGCAATGAAACCTTG
  ACACTGAAGCTGTACAACAATGGCTTTACTTCAGTCCAAGGATATGCTTTCAATGGGACA
  AAGCTGGATGCTGTTTACCTAAACAAGAATAAATACCTGACAGTTATTGACAAAGATGCA
  TTTGGAGGAGTATACAGTGGACCAAGCTTGCTGGACGTGTCTCAAACCAGTGTCACTGCC
  CTTCCATCCAAAGGCCTGGAGCACCTGAAGGAACTGATAGCAAGAAACACCTGGACTCTT
  AAGAAACTTCCACTTTCCTTGAGTTTCCTTCACCTCACACGGGCTGACCTTTCTTACCCA
  AGCCACTGCTGTGCTTTTAAGAATCAGAAGAAAATCAGAGGAATCCTTGAGTCCTTGATG
  TGTAATGAGAGCAGTATGCAGAGCTTGCGCCAGAGAAAATCTGTGAATGCCTTGAATAGC
  CCCCTCCACCAGGAATATGAAGAGAATCTGGGTGACAGCATTGTTGGGTACAAGGAAAAG
  TCCAAGTTCCAGGATACTCATAACAACGCTCATTATTACGTCTTCTTTGAAGAACAAGAG
  GATGAGATCATTGGTTTTGGCCAGGAGCTCAAAAACCCCCAGGAAGAGACTCTACAAGCT
  TTTGACAGCCATTATGACTACACCATATGTGGGGACAGTGAAGACATGGTGTGTACCCCC
  AAGTCCGATGAGTTCAACCCGTGTGAAGACATAATGGGCTACAAGTTCCTGAGAATTGTG
  GTGTGGTTCGTTAGTCTGCTGGCTCTCCTGGGCAATGTCTTTGTCCTGCTTATTCTCCTC
  ACCAGCCACTACAAACTGAACGTCCCCCGCTTTCTCATGTGCAACCTGGCCTTTGCGGAT
  TTCTGCATGGGGATGTACCTGCTCCTCATCGCCTCTGTAGACCTCTACACTCACTCTGAG
  TACTACAACCATGCCATCGACTGGCAGACAGGCCCTGGGTGCAACACGGCTGGTTTCTTC
  ACTGTCTTTGCAAGCGAGTTATCGGTGTATACGCTGACGGTCATCACCCTGGAGCGCTGG
  TATGCCATCACCTTCGCCATGCGCCTGGACCGGAAGATCCGCCTCAGGCACGCATGTGCC
  ATCATGGTTGGGGGCTGGGTTTGCTGCTTCCTTCTCGCCCTGCTTCCTTTGGTGGGAATA
  AGTAGCTATGCCAAAGTCAGTATCTGCCTGCCCATGGACACCGAGACCCCTCTTGCTCTG
  GCATATATTGTTTTTGTTCTGACGCTCAACATAGTTGCCTTCGTCATCGTCTGCTGCTGT
  TATGTGAAGATCTACATCACAGTCCGAAATCCGCAGTACAACCCAGGGGACAAAGATACC
  AAAATTGCCAAGAGGATGGCTGTGTTGATCTTCACCGACTTCATATGCATGGCCCCAATC
  TCATTCTATGCTCTGTCAGCAATTCTGAACAAGCCTCTCATCACTGTTAGCAACTCCAAA
  ATCTTGCTGGTACTCTTCTATCCACTTAACTCCTGTGCCAATCCATTCCTCTATGCTATT
  TTCACCAAGGCCTTCCAGAGGGATGTGTTCATCCTACTCAGCAAGTTTGGCATCTGTAAA
  CGCCAGGCTCAGGCATACCGGGGGCAGAGGGTTCCTCCAAAGAACAGCACTGATATTCAG
  GTTCAAAAGGTTACCCACGACATGAGGCAGGGTCTCCACAACATGGAAGATGTCTATGAA
  CTGATTGAAAACTCCCATCTAACCCCAAAGAAGCAAGGCCAAATCTCAGAAGAGTATATG
  CAAACGGTTTTGTAA
  
  Clone variation with respect to NM_000369.2
  2181 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000369 unedited
  CCCCCAGTTCAGATTTTGTAATACAACTTACTATAGNNGGCGGCCGCGCAATTCGGCACG
  AGGCAGGGGTGCAGGGCGACGGCTTCGGCCGCACCGCGGGCTAGCCAGGGCTGCGTGCCC
  GCCTCTGACCCTCAGCAGAGGTGTCTCTGGCCAGGAGGAGCTGAAGTTCTGCAGGACATT
  GGTCCGCCCGCGGACAGTCCACTCCGCGGGGACTTTCTCTGGATAAGGACCTCCTCCACA
  GTGGTGAGGTCACAGCCCCTTGGAGCCCTCCCTCTTCCCACCCCTCCCGCTCCCGGGTCT
  CCTTTGGCCTGGGGTAACCCGAGGTGCAGAGCTGAGAATGAGGCGATTTCGGAGGATGGA
  GAAATAGCCCCGAGTCCCGTGGAAAATGAGGCCGGCGGACTTGCTGCAGCTGGTGCTGCT
  GCTCGACCTGCCCAGGGACCTGGGCGGAATGGGGTGTTCGTCTCCACCCTGCGAGTGCCA
  TCAGGAGGAGGACTTCAGAGTCACCTGCAAGGATATTCAACGCATCCCCAGCTTACCGCC
  CAGTACGCAGACTCTGAAGCTTATTGAGACTCACCTGAGAACTATTCCAAGTCATGCATT
  TTCTAATCTGCCCAATATTTCCAGAATCTACGTATCTATAGATGTGACTCTGCAGCAGCT
  GGAATCACACTCCTTCTACAATTTGAGTAAAGTGACTCACATAGAAATTCGGAATACCAG
  GAACTTAACTTACATAGACCCTGATGCCCTCAAAGAGCTCCCCCTCCTTAAGTTCCTTGG
  CATTTTCAACACTGGACTTAACATGTTCCCTTGACCTGACCAAAGTTATTCCACTGATAT
  ATNCTTTATACTTNGAAATACAGACGACCCTTACATGACGTCAATCCCTGTGCATGCTTT
  TCAGGGACTATGCCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000369 unedited
  TTTGGNAGNAATATTTTCTGTTGTGAGTGCAAATTTTAGTAACAATTCTGTTGAAGTCAC
  TTTTCTAGGACTCTGTTTCCAAAGCAATTAGTTCAGCTATTCAAGATGAAACACATTATT
  TATCAAGATGTGATATTTTGAAATGTCACAGGAGAGAAACACATGACAAAAGGCAACACA
  TTTGTTTAATTTCATTAGTTACAAGCGAACTTCTAGGTAAGTCATGATTTTCGATGTTTA
  TAATATTTCTATTAAGATGTGCAGGTCTCAATGATAGTGATGAGTGGGTATGGCTGTGGA
  GAGATTCCAGGTGAGTCCAGCAGGGACCATCTGTGGGGACCCATTAACGACACTTCACAA
  TCTTAAAACAACAAAGAGCTTTTATTTCATGGTGTGGTTTATGAGCATTCAGAAGTAATG
  TCATCTCTGCTTGGAGACTTTTCTAGCAGGACATTAGGGGGCTATCATCACAGGTAACCT
  GGAGAGTACATCTGTAGATAAAGATCCTGACTATTTTAAGTAAAGAAGAATTATTGGGGA
  AAAGAAGCTTTATGTCCAGTGATCAACAGGGCTGGAAAGAGCTGGCAGTTTCAGAAAAGA
  AGAAACCTCTCTTGACAGCTTTAGTATCATACCGTGGACTGGAAATGGCAAAAATCATCC
  CTTAAGACAAGCGAAGTGTTTTTGAAGTGACTCTTCATAGTGAAGTAGCAACTCTAAATA
  GCCAAAATTTGCAACCATCGTTTACTCTTTTATATAAACACGAATCTGTTTTTGGCCTGA
  TNATTACAGCTGGGTAGATCTAGTAATAAATGAATGATTCAGATCCAGGCGAGTATGTTT
  ACTCAATTGAATTTTTGGCTCAGACTCATTATCAAAAAGTGAACAAGGAAAAATTCCTGG
  GTCATGTGTCTAGGCTCCAGGACTATGATCAGCACNCTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000369
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000369.2, NP_000360.2
• RefSeq Size: 4410 bp
• RefSeq ORF: 2295 bp
• Locus ID: 7253
• UniProt ID: P16473
• Cytogenetics: 14q31.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Autoimmune thyroid disease, Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).