HMGCS1 (NM_002130) Human Untagged Clone

CAT#: SC118803

HMGCS1 (untagged)-Human 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) (HMGCS1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HMGCS1
• Synonyms: HMGCS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118803 sequence for NM_002130 edited (data generated by NextGen Sequencing)
  ATGCCTGGATCACTTCCTTTGAATGCAGAAGCTTGCTGGCCAAAAGATGTGGGAATTGTT
  GCCCTTGAGATCTATTTTCCTTCTCAATATGTTGATCAAGCAGAGTTGGAAAAATATGAT
  GGTGTAGATGCTGGAAAGTATACCATTGGCTTGGGCCAGGCCAAGATGGGCTTCTGCACA
  GATAGAGAAGATATTAACTCTCTTTGCATGACTGTGGTTCAGAATCTTATGGAGAGAAAT
  AACCTTTCCTATGATTGCATTGGGCGGCTGGAAGTTGGAACAGAGACAATCATCGACAAA
  TCAAAGTCTGTGAAGACTAATTTGATGCAGCTGTTTGAAGAGTCTGGGAATACAGATATA
  GAAGGAATCGACACAACTAATGCATGCTATGGAGGCACAGCTGCTGTCTTCAATGCTGTT
  AACTGGATTGAGTCCAGCTCTTGGGATGGACGGTATGCCCTGGTAGTTGCAGGAGATATT
  GCTGTATATGCCACAGGAAATGCTAGACCTACAGGTGGAGTTGGAGCAGTAGCTCTGCTA
  ATTGGGCCAAATGCTCCTTTAATTTTTGAACGAGGGCTTCGTGGGACACATATGCAACAT
  GCCTATGATTTTTACAAGCCTGATATGCTATCTGAATATCCTATAGTAGATGGAAAACTC
  TCCATACAGTGCTACCTCAGTGCATTAGACCGCTGCTATTCTGTCTACTGCAAAAAGATC
  CATGCCCAGTGGCAGAAAGAGGGAAATGATAAAGATTTTACCTTGAATGATTTTGGCTTC
  ATGATCTTTCACTCACCATATTGTAAACTGGTTCAGAAATCTCTAGCTCGGATGTTGCTG
  AATGACTTCCTTAATGACCAGAATAGAGATAAAAATAGTATCTATAGTGGCCTGGAAGCC
  TTTGGGGATGTTAAATTAGAAGACACCTACTTTGATAGAGATGTGGAGAAGGCATTTATG
  AAGGCTAGCTCTGAACTCTTCAGTCAGAAAACAAAGGCATCTTTACTTGTATCAAATCAA
  AATGGAAATATGTACACATCTTCAGTATATGGTTCCCTTGCATCTGTTCTAGCACAGTAC
  TCACCTCAGCAATTAGCAGGGAAGAGAATTGGAGTGTTTTCTTATGGTTCTGGTTTGGCT
  GCCACTCTGTACTCTCTTAAAGTCACACAAGATGCTACACCGGGGTCTGCTCTTGATAAA
  ATAACAGCAAGTTTATGTGATCTTAAATCAAGGCTTGATTCAAGAACTGGTGTGGCACCA
  GATGTCTTCGCTGAAAACATGAAGCTCAGAGAGGACACCCATCATTTGGTCAACTATATT
  CCCCAGGGTTCAATAGATTCACTCTTTGAAGGAACGTGGTACTTAGTTAGGGTGGATGAA
  AAGCACAGAAGAACTTACGCTCGGCGTCCCACTCCAAATGATGACACTTTGGATGAAGGA
  GTAGGACTTGTGCATTCAAACATAGCAACTGAGCATATTCCAAGCCCTGCCAAGAAAGTA
  CCAAGACTCCCTGCCACAGCAGCAGAACCTGAAGCAGCTGTCATTAGTAATGGGGAACAT
  TAA
  
  Clone variation with respect to NM_002130.6
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002130 unedited
  GTGCATAATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACGAGGCCTCGT
  GCCGAATTCGGCACGAGGCGAAGGGGAGGCGCCGCGGACTGTCCTTTCGTGGCTCACTCC
  CTTTCCTCTGCTGCCGCTCGGTCACGCTTGCTCTTTCACCATGCCTGGATCACTTCCTTT
  GAATGCAGAAGCTTGCTGGCCAAAAGATGTGGGAATTGTTGCCCTTGAGATCTATTTTCC
  TTCTCAATATGTTGATCAAGCAGAGTTGGAAAAATATGATGGTGTAGATGCTGGAAAGTA
  TACCATTGGCTTGGGCCAGGCCAAGATGGGCTTCTGCACAGATAGAGAAGATATTAACTC
  TCTTTGCATGACTGTGGTTCAGAATCTTATGGAGAGAAATAACCTTTCCTATGATTGCAT
  TGGGCGGCTGGAAGTTGGAACAGAGACAATCATCGACAAATCAAAGTCTGTGAAGACTAA
  TTTGATGCAGCTGTTTGAAGAGTCTGGGAATACAGATATAGAAGGAATCGACACAACTAA
  TGCATGCTATGGAGGCACAGCTGCTGTCTTCAATGCTGTTAACTGGATTGAGTCCAGCTC
  TTGGGATGGACGGTATGCCCTGGTAGTTGCAGGAGATATTGCTGTATATGCCACAGGAAA
  TGCTAGACCTACAGGTGGAGTTGGAGCAGTAGCTCTGCTAATTGGGCCAAATGCTCCTTT
  AATTTTTGAACGANGGCTTCGTGNGACACATATGCCACATGCCTATGATTNTTACAAGCC
  TGATATGCTATCTGAATATCCTATAGTAGATGGAAAACTCTCCTACAGTGCTACCTCAGT
  GCATTAGCCGCTGCTATCCTGTCTACTGCAAAAAGACCATGCCCAGTGCCAGAAAGAAGG
  GAATGATACAGATTTACCCTGCATGAATNTGGGCTCAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002130 unedited
  CCGCGGTCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGATTTATCAATTTTA
  GAAAAAGGGCATGTTTGTTTTGCTATTTTCAAAGGGTTTGTGCGTATCACATTTTACCAC
  ATTTTACCCCAATTTTACCACACTGGGGAATGTTGTCACATTCCCCAAGCTAGAAATGTT
  TTCCAAAAACCACAGGTACTTTCTGTAATCTTGTTGTTCAAAGTACAAAATTCTTACATG
  TTAAAAAATTTTTTAATTGGAGATTAGTACCTCTGCTTTCTTCTGACAGAAGTACACAAT
  TCATTATACCATGTTTAAAAACCAAACATCATGCATACAAATGGCCAAAGAGGTATGAAG
  TTTTACAGAGCCCTTAACATCATTCGAGTACATTTGGCATTGGCCAGACCACAACAGGAA
  GCATGTCAGCAAATAGAGCAAAGAGACTNTCCCAAGTACACGATAGTCATCCAGGCTCCA
  TGTCAGTCACATAAAAATTTACATAACATGTAATCCTTTAAAATTATCCCCAGATATCCC
  ATTCCTTCAACTGTTCCCATACCCCNCACCCCATGCCCACCCCCACCCTTGAGTCCTTGC
  CCCTCACAGAGTATCTTTATTGTTCCCCATTACTAATGACAGCTGCTTTCAGTTCTGCTG
  CTGTGGCAGGGAGTCCTGGTACTTTCCTGCCACGGCTCGGAATATCTCAGCTGCTATTTT
  GAATGCCAATTCCTACTCTTTTTCCAAGTGCATCTTTTGGAGTGGACCCCCACCGTAGTT
  CTTCTGTGCCTTTCATCCCCCTTAACTAAAACCCACGTTCCTTAAAAGAAGAATCTTTGA
  CCCTGGGGATAATACTACCAATGATGGCGCGCCTTTTGGATTTTGTTTTAAGCAAACATT
  TGTGCCCACCACTCCTGATTAAGCCTTGTTAAAATAATAACTTTCGTATTAAAAGAGACA
  ACCGTGGACACCTTTGGTACTTCAAAGGACATAGGGCCCCCCCCCACCTTA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002130
• Insert Size: 2260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002130.4, NP_002121.3
• RefSeq Size: 3421 bp
• RefSeq ORF: 1563 bp
• Locus ID: 3157
• UniProt ID: Q01581
• Cytogenetics: 5p12
• Domains: HMG_CoA_synt
• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies, Terpenoid backbone biosynthesis, Valine, leucine and isoleucine degradation
• Gene Summary: This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.[UniProtKB/Swiss-Prot Function]