ABCG1 (NM_004915) Human Untagged Clone

CAT#: SC117061

ABCG1 (untagged)-Human ATP-binding cassette, sub-family G (WHITE), member 1 (ABCG1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCG1
• Synonyms: ABC8; WHITE1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004915, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTGTCTGATGGCCGCTTTCTCGGTCGGCACCGCCATGAATGCCAGCAGTTACTCTGCAGAGATGA
  CGGAGCCCAAGTCGGTGTGTGTCTCGGTGGATGAGGTGGTGTCCAGCAACATGGAGGCCACTGAGACGGA
  CCTGCTGAATGGACATCTGAAAAAAGTAGATAATAACCTCACGGAAGCCCAGCGCTTCTCCTCCTTGCCT
  CGGAGGGCAGCTGTGAACATTGAATTCAGGGACCTTTCCTATTCGGTTCCTGAAGGACCCTGGTGGAGGA
  AGAAAGGATACAAGACCCTCCTGAAAGGAATTTCCGGGAAGTTCAATAGTGGTGAGTTGGTGGCCATTAT
  GGGTCCTTCCGGGGCCGGGAAGTCCACGCTGATGAACATCCTGGCTGGATACAGGGAGACGGGCATGAAG
  GGGGCCGTCCTCATCAACGGCCTGCCCCGGGACCTGCGCTGCTTCCGGAAGGTGTCCTGCTACATCATGC
  AGGATGACATGCTGCTGCCGCATCTCACTGTGCAGGAGGCCATGATGGTGTCGGCACATCTGAAGCTTCA
  GGAGAAGGATGAAGGCAGAAGGGAAATGGTCAAGGAGATACTGACAGCGCTGGGCTTGCTGTCTTGCGCC
  AACACGCGGACCGGGAGCCTGTCAGGTGGTCAGCGCAAGCGCCTGGCCATCGCGCTGGAGCTGGTGAACA
  ACCCTCCAGTCATGTTCTTCGATGAGCCCACCAGCGGCCTGGACAGCGCCTCCTGCTTCCAGGTGGTCTC
  GCTGATGAAAGGGCTCGCTCAAGGGGGTCGCTCCATCATTTGCACCATCCACCAGCCCAGCGCCAAACTC
  TTCGAGCTGTTCGACCAGCTTTACGTCCTGAGTCAAGGACAATGTGTGTACCGGGGAAAAGTCTGCAATC
  TTGTGCCATATTTGAGGGATTTGGGTCTGAACTGCCCAACCTACCACAACCCAGCAGATTTTGTCATGGA
  GGTTGCATCCGGCGAGTACGGTGATCAGAACAGTCGGCTGGTGAGAGCGGTTCGGGAGGGCATGTGTGAC
  TCAGACCACAAGAGAGACCTCGGGGGTGATGCCGAGGTGAACCCTTTTCTTTGGCACCGGCCCTCTGAAG
  AGGTAAAGCAGACAAAACGATTAAAGGGGTTGAGAAAGGACTCCTCGTCCATGGAAGGCTGCCACAGCTT
  CTCTGCCAGCTGCCTCACGCAGTTCTGCATCCTCTTCAAGAGGACCTTCCTCAGCATCATGAGGGACTCG
  GTCCTGACACACCTGCGCATCACCTCGCACATTGGGATCGGCCTCCTCATTGGCCTGCTGTACTTGGGGA
  TCGGGAACGAAGCCAAGAAGGTCTTGAGCAACTCCGGCTTCCTCTTCTTCTCCATGCTGTTCCTCATGTT
  CGCGGCCCTCATGCCTACTGTTCTGACATTTCCCCTGGAGATGGGAGTCTTTCTTCGGGAACACCTGAAC
  TACTGGTACAGCCTGAAGGCCTACTACCTGGCCAAGACCATGGCAGACGTGCCCTTTCAGATCATGTTCC
  CAGTGGCCTACTGCAGCATCGTGTACTGGATGACGTCGCAGCCGTCCGACGCCGTGCGCTTTGTGCTGTT
  TGCCGCGCTGGGCACCATGACCTCCCTGGTGGCACAGTCCCTGGGCCTGCTGATCGGAGCCGCCTCCACG
  TCCCTGCAGGTGGCCACTTTCGTGGGCCCAGTGACAGCCATCCCGGTGCTCCTGTTCTCGGGGTTCTTCG
  TCAGCTTCGACACCATCCCCACGTACCTACAGTGGATGTCCTACATCTCCTATGTCAGGTATGGGTTCGA
  AGGGGTCATCCTCTCCATCTATGGCTTAGACCGGGAAGATCTGCACTGTGACATCGACGAGACGTGCCAC
  TTCCAGAAGTCGGAGGCCATCCTGCGGGAGCTGGACGTGGAAAATGCCAAGCTGTACCTGGACTTCATCG
  TACTCGGGATTTTCTTCATCTCCCTCCGCCTCATTGCCTATTTTGTCCTCAGGTACAAAATCCGGGCAGA
  GAGGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004915 unedited
  NCGGTTTCGATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCAGCCC
  GCACCCCGCGCAGCGGCTGAGCCGGGAGCCAGCGCAGCCTCGGCCCCGCAGCTCAAGCCT
  CGTCCCCGCCGCCGCCGCCGCCGCCGCCGCCGCCGCCCCCGGGGCATGGCCTGTCTGATG
  GCCGCTTTCTCGGTCGGCACCGCCATGAATGCCAGCAGTTACTCTGCAGAGATGACGGAG
  CCCAAGTCGGTGTGTGTCTCGGTGGATGAGGTGGTGTCCAGCAACATGGAGGCCACTGAG
  ACGGACCTGCTGAATGGACATCTGAAAAAAGTAGATAATAACCTCACGGAAGCCCAGCGC
  TTCTCCTCCTTGCCTCGGAGGGCAGCTGTGAACATTGAATTCAGGGACCTTTCCTATTCG
  GTTCCTGAAGGACCCTGGTGGAGGAAGAAAGGATACAAGACCCTCCTGAAAGGAATTTCC
  GGGAAGTTCAATAGTGGTGAGTTGGTGGCCATTATGGGTCCTTCCGGGGCCGGGAAGTCC
  ACGCTGATGAACATCCTGGCTGGATACAGGGAGACGGGCATGAAGGGGGCCGTCCTCATC
  AACGGCCTGCCCCGGGACCTGCGCTGCTTCCGNGAGGTGTCCTGCTACATCATGCANGAT
  GACATGCTGCTGCCGCATCTCACTGTGCAGGAGGCCATGATGGTGTCGGCACATCTGAAG
  CTTCAGGAGAAGGATGAAGGCAGAAGGGAAATGGTCAAGGAGATACTGACAGCGCTGGGC
  TTGCTGTCTTGCGCCACACGCGGACCGNGAGCCTGTCAGGTGGTCAGCGCAAGCGCCTGG
  CCATCGCGCTGGAGCTGGGTGACNACCCTNNCAGTCATGTCTNCGATGAGCCCNACCAGC
  GCCTGGACAGCGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004915 unedited
  AAAACATCTCATTTACGCTTGAAAATAAGTAGTCACGTGGGAAAAGTAAGACTTTGAAAG
  AGTTGGCCAATGTCTTCTCACAATTTTTTGTATAGCTTTTAAAAATGTCTGCATGGCTCA
  TTGGAGATGGTGTCCACGTCTTCCCTCCACCTGGAACCTGCATTCAATCCTTCACAAGTG
  CTGTCTATGTATGAGACCAGCTGGGTGACTCGGTTAAACCCCTCGCCTCTCTAAACAAAG
  TATGTGATTTACCCTGAATCATTCTTCAGCAAGTACTAAAAAGGTATAATTTCTTAAAAT
  GAATGCGATTGATCGACTTTTGGCTTGCAAAAATGACTTCTCATTGGAAAAGAGACACAC
  CTTAGAAAGATGAAAATGTGAAAAACAACAGTCATAAAATGGAATCGCTCCATGCTCGGA
  CTCTCTGCCACCAGACATCTGACTGTGCAGCCATCAGCTGAGAGGCTGCTTGCAGTTCCC
  CATCCAGGACCCACGGTGCCTGGTGCTAAGGACCGACTGGACTGAGGAAGCCTCTCATCA
  CCAGCTGTGTCGCAGCTTTAAATTCTGCCCCAGTTGTGGCATTTAAAATCCTGATCGTAA
  AAAAAAAAAAAAAACACCCATCTGCCCACCTCTTCCTAGCTAAAGCTAGAAAGGGGAATG
  GGAGAGAATACCCACCCCACCTTGGCCTAGTGGCTGAGCTCGATAAACCCCCAAACCCCA
  ACGCGGTTCCAAGGGGTTGGGTTCAAAAAATTTCCTGGTCGCCCCGTCGGGGACAAGGCT
  TGCCCTCCCCCATTCTCAGTCCGCGCCCCTCGTCCTCCATGTTCCTATCCTTCTTGTTTC
  CTGGGCCTACACGGCGTTTTCCCCTTCCTCTCGTTCTTATGTTCCCACGCATTAATTCTC
  CCCCTTCCTCTACGCGTCCTCAAACAATTTCCACTCCGCGTATTACTTCTCTGTCATCTC
  CGGCTGTNTCCTCGCGCATCCCGCTTGTGTTCTCTTCCTCTCTCCACGTTGTTGCCTTTG
  TCGCCTCGCCTCTCGTATTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004915
• Insert Size: 3270 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004915.3, NP_004906.3
• RefSeq Size: 3018 bp
• RefSeq ORF: 2037 bp
• Locus ID: 9619
• UniProt ID: P45844
• Cytogenetics: 21q22.3
• Domains: ABC_tran, AAA
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types. Six alternative splice variants have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (4) differs in the 5' end-region compared to variant 1, which includes a part of the coding region. The resulting isoform (4) has a distinct and shorter N-terminus, as compared to isoform 1.