Perforin (PRF1) (NM_005041) Human Untagged Clone

CAT#: SC116957

PRF1 (untagged)-Human perforin 1 (pore forming protein) (PRF1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Perforin
• Synonyms: HPLH2; P1; PFP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116957 sequence for NM_005041 edited (data generated by NextGen Sequencing)
  ATGGCAGCCCGTCTGCTCCTCCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCT
  GCCCCGTGCCACACAGCCGCACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGT
  GCATGGCTGGCCGGGGAGGGTGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCA
  GTGGACACACAAAGGTTCCTGCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTA
  CAGGAGGGCACCCTCCAGCGCCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCT
  GGCTGCCAGCGCCATGTAACCAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGAT
  GCGGCTCGTAGCATCCGCAACGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACC
  AGCAATGTGCATGTGTCTGTGGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAG
  AAGACCCACCAGGACCAGTACAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGT
  TTCCATGTGGTACACACTCCCCCGCTGCACCCTGACTTCAAGAGGGCCCTCGGGGACCTG
  CCCCACCACTTCAACGCCTCCACCCAGCCCGCCTACCTCAGGCTTATCTCCAACTACGGC
  ACCCACTTCATCCGGGCTGTGGAGCTGGGTGGCCGCATATCGGCCCTCACTGCCCTGCGC
  ACCTGCGAGCTGGCCCTGGAAGGGCTCACGGACAACGAGGTGGAGGACTGCCTGACTGTC
  GAGGCCCAGGTCAACATAGGCATCCACGGCAGCATCTCTGCCGAAGCCAAGGCCTGTGAG
  GAGAAGAAGAAGAAGCACAAGATGACGGCCTCCTTCCACCAAACCTACCGGGAGCGCCAT
  TCGGAAGTGGTTGGCGGCCATCACACCTCCATTAACGACCTGCTGTTCGGGATCCAGGCC
  GGGCCCGAGCAGTACTCAGCCTGGGTAAACTCGCTGCCCGGCAGCCCTGGCCTGGTGGAC
  TACACCCTGGAACCCCTGCACGTGCTGCTGGACAGCCAGGACCCGCGGCGGGAGGCACTG
  AGGAGGGCCCTGAGTCAGTACCTGACGGACAGGGCTCGCTGGAGGGACTGCAGCCGGCCG
  TGCCCACCAGGGCGGCAGAAGAGCCCCCGAGACCCATGCCAGTGTGTGTGCCATGGCTCA
  GCGGTCACCACCCAGGACTGCTGCCCTCGGCAGAGGGGCCTGGCCCAGCTGGAGGTGACC
  TTCATCCAAGCATGGGGCCTGTGGGGGGACTGGTTCACTGCCACGGATGCCTATGTGAAG
  CTCTTCTTTGGTGGCCAGGAGCTGAGGACGAGCACCGTGTGGGACAATAACAACCCCATC
  TGGTCAGTGCGGCTGGATTTTGGGGATGTGCTCCTGGCCACAGGGGGGCCCCTGAGGTTG
  CAGGTCTGGGATCAGGACTCTGGCAGGGACGATGACCTCCTTGGCACCTGTGATCAGGCT
  CCCAAGTCTGGTTCCCATGAGGTGAGATGCAACCTGAATCATGGCCACCTAAAATTCCGC
  TATCATGCCAGGTGCTTGCCCCACCTGGGAGGAGGCACCTGCCTGGACTATGTCCCCCAA
  ATGCTTCTGGGGGAGCCTCCAGGAAACCGGAGTGGGGCCGTGTGGTGA
  
  Clone variation with respect to NM_005041.4
  900 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005041 unedited
  TTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTGAAGAGAGGATAT
  CCATCTGTGTAGCCGCTTCTCTATACGGGATTCCAGCTCCATGGCAGCCCGTCTGCTCCT
  CCTGGGCATCCTTCTCCTGCTGCTGCCCCTGCCCGTCCCTGCCCCGTGCCACACAGCCGC
  ACGCTCAGAGTGCAAGCGCAGCCACAAGTTCGTGCCTGGTGCATGGCTGGCCGGGGAGGG
  TGTGGACGTGACCAGCCTCCGCCGCTCGGGCTCCTTCCCAGTGGACACACAAAGGTTCCT
  GCGGCCCGACGGCACCTGCACCCTCTGTGAAAATGCCCTACAGGAGGGCACCCTCCAGCG
  CCTGCCTCTGGCGCTCACCAACTGGCGGGCCCAGGGCTCTGGCTGCCAGCGCCATGTAAC
  CAGGGCCAAAGTCAGCTCCACTGAAGCTGTGGCCCGGGATGCGGCTCGTAGCATCCGCAA
  CGACTGGAAGGTCGGGCTGGACGTGACTCCTAAGCCCACCAGCAATGTGCATGTGTCTGT
  GGCCGGCTCACACTCACAGGCAGCCAACTTTGCAGCCCAGAAGACCCACCAGGACCAGTA
  CAGCTTCAGCACTGACACGGTGGAGTGCCGCTTCTACAGTTTCCATGTGGTACACACTCC
  CCCGCTGCACCCTGACTTCAAGAGGGCCCTNCGNGACCTGCCCCACCACTTAAACGCCTC
  CACCCAGCCNGNCTACCTNAGGCTATCTNCAACTACGGGACCCACTTCATCCGGGCTGTG
  GAGCTGGGTGGCCGATATCGGCCCTCACTGCCCTCGCACCTGCGAGCTGGCCCTGGNAAG
  GCTCACCGNACAACGAGTGNAGGACTGNCTGACTGTCGAGCCCCAGTTAACATAAGCATT
  CCCGGAGAATTTCTGCCGAAACAAAGCCTGTGAGAAAAAANAATAAGCACAGATGACGCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005041 unedited
  ACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCACCATAACATCATAT
  TTATTGGCCCTTTATCAAGCTATGTACATGGTGAGACAGGTCAGGACAGGCCTCCATTTG
  TCTCAGGGGCAAAGCATTGTGGGCAAAGAAGACAGAGCAGCTGGAGCTGATGGGGCTCCA
  GTTAAGGCAATGAAGGCTTTGCCACACCATAGAGGGCTCAAGGGAAGGGTCCTAAAAGAC
  CAGCCCTGAGCAGCCTGGTGGGAACAGCCTCTTGGCCTTCTGCCCAGCTCCTGGCTGAAG
  CTGTGATCTGTGTAGCTGTGACTGCAGGGCTTGAGAATGGCGGAGGGCTTAGGCAGTTTG
  GACAGGGTGAATCGGGATTAGCGTGTAAACCCAGCCACCTCCCTGAAAAACAGTCTGAAT
  CTCCCTTTTCCATCTGTCTGATGCGTATCCAATCTTTTGGCTTCTCTGGGCCACGTTGGA
  AGAAGAATTGTGTTGGGCCACATGTAAAATCCACTAGCACTAACGATAGCCGATGAGCTA
  AAAAAAAAAAAAAATAGCAAAAAGAAACTCATAATGTTTTAAGAAAGTTTGCGAATTTGC
  GTTGGGCCGCATTCAAAGCCATCCTGGGCCGCATGCGGGCCTCGGGTTGGATAAGCTTGG
  TCTAATGGGAATACGAGGACAGCCTTGGCTCCCACTGTGAGAACCCCTTCAGTCCAAGCA
  TACTGGTCCTTTNTCAGCTCACTGTTCTCACCACCCGGCCCCACCTCCGTTTTCCTGGAA
  GCCTCCCCCAGAAGCATTTGGGGGACATAGTCCAGGCAGGTCGCTTCCTCCCAGTGGGGC
  AAGCACCCTGCATGATAGCGAATTTTTAGGTGGCCTGGATCACGTTGCATCTCACCTCAT
  GGAACCAAACTGTGGACCCTGATCACAGTGCCAAGGAGTCATTGTCCCTGGCAAAACCTG
  ATCCAGACCGGAACCTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005041
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005041.3, NP_005032.2
• RefSeq Size: 2512 bp
• RefSeq ORF: 1668 bp
• Locus ID: 5551
• UniProt ID: P14222
• Cytogenetics: 10q22.1
• Domains: C2, MACPF
• Protein Families: Druggable Genome
• Protein Pathways: Allograft rejection, Autoimmune thyroid disease, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis
• Gene Summary: This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
  Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein.