c-Myb (MYB) (NM_005375) Human Untagged Clone

CAT#: SC116779

MYB (untagged)-Human v-myb myeloblastosis viral oncogene homolog (avian) (MYB), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: c-Myb
• Synonyms: c-myb; c-myb_CDS; Cmyb; efg
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005375, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCGAAGACCCCGGCACAGCATATATAGCAGTGACGAGGATGATGAGGACTTTGAGATGTGTGACC
  ATGACTATGATGGGCTGCTTCCCAAGTCTGGAAAGCGTCACTTGGGGAAAACAAGGTGGACCCGGGAAGA
  GGATGAAAAACTGAAGAAGCTGGTGGAACAGAATGGAACAGATGACTGGAAAGTTATTGCCAATTATCTC
  CCGAATCGAACAGATGTGCAGTGCCAGCACCGATGGCAGAAAGTACTAAACCCTGAGCTCATCAAGGGTC
  CTTGGACCAAAGAAGAAGATCAGAGAGTGATAGAGCTTGTACAGAAATACGGTCCGAAACGTTGGTCTGT
  TATTGCCAAGCACTTAAAGGGGAGAATTGGAAAACAATGTAGGGAGAGGTGGCATAACCACTTGAATCCA
  GAAGTTAAGAAAACCTCCTGGACAGAAGAGGAAGACAGAATTATTTACCAGGCACACAAGAGACTGGGGA
  ACAGATGGGCAGAAATCGCAAAGCTACTGCCTGGACGAACTGATAATGCTATCAAGAACCACTGGAATTC
  TACAATGCGTCGGAAGGTCGAACAGGAAGGTTATCTGCAGGAGTCTTCAAAAGCCAGCCAGCCAGCAGTG
  GCCACAAGCTTCCAGAAGAACAGTCATTTGATGGGTTTTGCTCAGGCTCCGCCTACAGCTCAACTCCCTG
  CCACTGGCCAGCCCACTGTTAACAACGACTATTCCTATTACCACATTTCTGAAGCACAAAATGTCTCCAG
  TCATGTTCCATACCCTGTAGCGTTACATGTAAATATAGTCAATGTCCCTCAGCCAGCTGCCGCAGCCATT
  CAGAGACACTATAATGATGAAGACCCTGAGAAGGAAAAGCGAATAAAGGAATTAGAATTGCTCCTAATGT
  CAACCGAGAATGAGCTAAAAGGACAGCAGGTGCTACCAACACAGAACCACACATGCAGCTACCCCGGGTG
  GCACAGCACCACCATTGCCGACCACACCAGACCTCATGGAGACAGTGCACCTGTTTCCTGTTTGGGAGAA
  CACCACTCCACTCCATCTCTGCCAGCGGATCCTGGCTCCCTACCTGAAGAAAGCGCCTCGCCAGCAAGGT
  GCATGATCGTCCACCAGGGCACCATTCTGGATAATGTTAAGAACCTCTTAGAATTTGCAGAAACACTCCA
  ATTTATAGATTCTTTCTTAAACACTTCCAGTAACCATGAAAACTCAGACTTGGAAATGCCTTCTTTAACT
  TCCACCCCCCTCATTGGTCACAAATTGACTGTTACAACACCATTTCATAGAGACCAGACTGTGAAAACTC
  AAAAGGAAAATACTGTTTTTAGAACCCCAGCTATCAAAAGGTCAATCTTAGAAAGCTCTCCAAGAACTCC
  TACACCATTCAAACATGCACTTGCAGCTCAAGAAATTAAATACGGTCCCCTGAAGATGCTACCTCAGACA
  CCCTCTCATCTAGTAGAAGATCTGCAGGATGTGATCAAACAGGAATCTGATGAATCTGGAATTGTTGCTG
  AGTTTCAAGAAAATGGACCACCCTTACTGAAGAAAATCAAACAAGAGGTGGAATCTCCAACTGATAAATC
  AGGAAACTTCTTCTGCTCACACCACTGGGAAGGGGACAGTCTGAATACCCAACTGTTCACGCAGACCTCG
  CCTGTGGCAGATGCACCGAATATTCTTACAAGCTCCGTTTTAATGGCACCAGCATCAGAAGATGAAGACA
  ATGTTCTCAAAGCATTTACAGTACCTAAAAACAGGTCCCTGGCGAGCCCCTTGCAGCCTTGTAGCAGTAC
  CTGGGAACCTGCATCCTGTGGAAAGATGGAGGAGCAGATGACATCTTCCAGTCAAGCTCGTAAATACGTG
  AATGCATTCTCAGCCCGGACGCTGGTCATGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005375 unedited
  GGTTAAAATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGGCAGGCAGG
  CGGCGGGCAGCGGGAGGCGGCAGCCCGGTGCGGTCCCCGCGGCTCTCGGCGGAGCCCCGC
  GCCCGCCGCGCCATGGCCCGAAGACCCCGGCACAGCATATATAGCAGCGACGAGGATGAT
  GAGGACTTTGAGATGTGTGACCATGACTATGATGGGCTGCTTCCCAAGTCTGGAAAGCGT
  CACTTGGGGAAAACAAGGTGGACCCGGGAAGAGGATGAAAAACTGAAGAAGCTGGTGGAA
  CAGAATGGAACAGATGACTGGAAAGTTATTGCCAATTATCTCCCGAATCGAACAGATGTG
  CAGTGCCAGCACCGATGGCAGAAAGTACTAAACCCTGAGCTCATCAAGGGTCCTTGGACC
  AAAGAAGAAGATCAGAGAGTGATAGAGCTTGTACAGAAATACGGTCCGAAACCGTTGGTC
  TGTTATTGCCCAAGCCACCTTAAAGGGGAGAATTGGAAAACAATGTATGGAGAGGTGGCA
  TAACCACTTGAATCCACAAGTTAAGAAAACCTCCTGGACCGAACAGGAAGACAGAATTAT
  TTACCAGGCACACCAGAGACTGGGGGAACAGAATGGCCACACATCGCAAAGCTACTGCCT
  GGCACGAACTGATCATGCTATCCAAGAACCACTGGGCAATTCTACAATGCCTCCCAAAGG
  CCCACCAGGAAGGTTATCTTGAGCACTTTTCAAAGCCCCCCCGCCCGCAGTGCCCCCATC
  TTTCACACAACAACCTTCGATGGCTTTTGCTCAGCTCCGCCTACAGCTTACTTCCTGCCC
  TTGGCCACCCACCGTTACCCCCACTATTCCTATACCCCTTTTTGAGCCCCAACGTCTCCA
  CCCTGCTCCATCCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005375 unedited
  CGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTAAAATAAGGGCAC
  ATCTTTATTTTGCTACAAGGCAGTAAGTACACCGTCATATCTCAAAAGTTCAGTGCTGGC
  CATCTTGCATCAAATGTTCTTAAGGCAGTGACTGGCTATCAACCACAGTTTCTGTCTCCC
  CAGTTGCAAACACAGGATCCATGCAACAGTTCTGAGACCATACACTTAGAAACCACAGGG
  GATGCGGATCAAATGCAGAACTCCCAAATTATAAAACAGTCAGGCTACACTCAAAACAAA
  ACATAGAACATCAACAACACACATCTCCCAAAAAAGAAGTGCAACGCATGCTTGTATAAA
  CCAACAATAACAAAAAAACCACAATAAAAAATGCAGAGTCTCCCAAACAAGTTTTCAAAT
  GTATTGCAGAAGGAAAAAAAATGTATATATATATAAAATTAAAAAGTCTGAAATACTAGT
  GCATAGTCAATTACCTAACACCAAGTTTCTTTTCTTTCTGTCCAAGCTCTACTGCCCCTC
  TGATACTAGCAGCATGTCTACAGGCTAAGACCATAGCAGCAAAAAACGTTTTTCATTTGG
  CATTTACAAAATTAAATTACTGAATAAAAATATAATTTTTTATAAAACTATTTCTTACAG
  TAATAATTTTTAAAGCAAGCTAACAGAAAACTCATAAAATGGTTTGGTACAATAAATGTA
  CCTTCAATGTCATTAACCATAAATGAGCATTTACCATCTGGATTAAATGTCACATGGTAT
  TAAGTCTACACTTANAGTAATGCTCTTACTGATTTCTAAAATATATGCATATGCCTAGTG
  ATCGAGAAAGTGAAATACTGGAGTACCCTTTAATGTGTCAAATTTCAAAAAACCATCACA
  GGAAAAATACTGTCTGTTGGACCCTTAAATACAGATAATCACTTTAGTTTTTTTTAAAAA
  ACTGCCTAAGAATTACACTGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005375
• Insert Size: 3180 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005375.2, NP_005366.2
• RefSeq Size: 3342 bp
• RefSeq ORF: 1923 bp
• Locus ID: 4602
• UniProt ID: P10242
• Cytogenetics: 6q23.3
• Domains: myb_DNA-binding
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency, Transcription Factors
• Gene Summary: This gene encodes a protein with three HTH DNA-binding domains that functions as a transcription regulator. This protein plays an essential role in the regulation of hematopoiesis. This gene may be aberrently expressed or rearranged or undergo translocation in leukemias and lymphomas, and is considered to be an oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2, also known as M15024) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) is shorter than isoform 1.