BCKDH kinase (BCKDK) (NM_005881) Human Untagged Clone

CAT#: SC116464

BCKDK (untagged)-Human branched chain ketoacid dehydrogenase kinase (BCKDK), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCKDH kinase
• Synonyms: BCKDKD; BDK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005881 edited
  ATGATCCTGGCGTCGGTGCTGAGGAGCGGTCCCGGGGGCGGGCTTCCGCTCCGGCCCCTC
  CTGGGACCCGCACTCGCGCTCCGGGCCCGCTCGACGTCGGCCACCGACACACACCACGTG
  GAGATGGCTCGGGAGCGCTCCAAGACCGTCACCTCCTTTTACAACCAGTCGGCCATCGAC
  GCGGCAGCGGAGAAGCCCTCAGTCCGCCTAACGCCCACCATGATGCTCTACGCTGGCCGC
  TCTCAGGACGGCAGCCACCTTCTGAAAAGTGCTCGGTACCTGCAGCAAGAACTTCCAGTG
  AGGATTGCTCACCGCATCAAGGGCTTCCGCTGCCTTCCTTTCATCATTGGCTGCAACCCC
  ACCATACTGCACGTGCATGAGCTATATATCCGTGCCTTCCAGAAGCTGACAGACTTCCCT
  CCGATCAAGGACCAGGCGGACGAGGCCCAGTACTGCCAGCTGGTGCGACAGCTGCTGGAT
  GACCACAAGGATGTGGTGACCCTCTTGGCAGAGGGCCTACGTGAGAGCCGGAAGCACATA
  GAGGATGAAAAGCTCGTCCGCTACTTCTTGGACAAGACGCTGACTTCGAGGCTTGGAATC
  CGCATGTTGGCCACACATCACCTGGCGCTGCATGAGGACAAGCCTGACTTTGTCGGCATC
  ATCTGTACTCGTCTCTCACCAAAGAAGATTATTGAGAAGTGGGTGGACTTTGCCAGACGC
  CTGTGTGAGCACAAGTATGGCAATGCGCCCCGTGTCCGCATCAATGGCCATGTGGCTGCC
  CGGTTCCCCTTCATCCCTATGCCACTGGACTACATCCTGCCGGAGCTGCTCAAGAATGCC
  ATGAGAGCCACAATGGAGAGTCACCTAGACACTCCCTACAATGTCCCAGATGTGGTCATC
  ACCATCGCCAACAATGATGTCGATCTGATCATCAGGATCTCAGACCGTGGTGGAGGAATC
  GCTCACAAAGATCTGGACCGGGTCATGGACTACCACTTCACTACTGCTGAGGCCAGCACA
  CAGGACCCCCGGATCAGCCCCCTCTTTGGCCATCTGGACATGCATAGTGGCGCCCAGTCA
  GGACCCATGCACGGCTTTGGCTTCGGGTTGCCCACGTCACGGGCCTACGCGGAGTACCTC
  GGTGGGTCTCTGCAGCTGCAGTCCCTGCAGGGCATTGGCACGGACGTCTACCTGCGGCTC
  CGCCACATCGATGGCCGGGAGGAAAGCTTCCGGATCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005881 unedited
  TCATATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACCAGGTTCGAGCCCTTC
  CGCTGGGACCCGGGCCCTGGCTCCGGCCCCGCGATGGGAGCTGCTCTCCGCGGGCTGAGC
  CTGTCAGCATCCTCGACGCACCCTGGTCCCTGAAGTCGGAGAAGAGCCCCTACCCACCCA
  CACCCCCTTGCCCCATTTTGGGTCGCCTGGGTCCTCAGTCCTAGCGGATCCTCAGTCCTA
  GCGGCCACCGGGTCTGAAAGGAGCAAGACGATGATCCTGGCGTCGGTGCTGAGGAAGCGG
  TCCCGGGGGCGGGCTTCCGCTCCGGCCCCTCCTGGGACCCGCACTCGCGCTCCGGGCCCG
  CTCGACGTCGGCCACCGACACACACCACGTGGAGATGGCTCGGGAGCGCTCCAAGACCGT
  CACCTCCTTTTACAACCAGTCGGCCATCGACGCGGCAGCGGAGAAGCCCTCAGTCCGCCT
  AACGCCCACCATGATGCTCTACGCTGGCCGCTCTCAGGACGGCAGCCACCTTCTGAAAAG
  TGCTCGGTACCTGCAGCAAGAACTTCCAGTGAGGATTGCTCACCGCATCAAGGGCTTCCG
  CTGCCTTCCTTTCATCATTGGCTGCAACCCCACCATACTGCACGTGCATGAGCTATATAT
  CCGTGCCTTCCAGAAGCTGACAGACTTCCCTCCGATCAAGGACCAGGCGGACGAGGCCCA
  GTACTGCCAGCTGGTGCGACAGCTGCTGGATGACCACAAGATGTGGTGACCCTCTTGGCA
  GAGGGCCTACGTGAGAGCCCGGAGCCATAGAGGATGAAAAGCTCGTCCGCTACTTCTTGG
  ACNAGACGCTGACTTCGAGGCTTGGAATCCGCATGTTGGCCACACATCACCTGGCGCTGC
  ATGAGGACAAGCCTGACTTTGTCGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005881 unedited
  TTTAAGGTTTCTACTATGNACCGCGGCACGCAATCTATGATCGATTTTTTTTTTTTTTTT
  TTTTTTTTGGGCATTCAAAAGTGAACTTTAATAACAGGTCAGAGTGGGGGTTCCCCAGGA
  ACGAGAATGACGGAACTGGTGCTGGAGGCCTCAAGGTGCCCACTTCCCTGCTCCAAGTTC
  TGGAGGCGAGGAGGCAATGGACCCTGTTGAGGCAGGGCAGTGCCCAGCTCCATGTAAGTC
  CATCTGTCTGGGTCCCTGAGACCCAAGATGCAGCAGTGTGTGGAGCAAGGGGCCGCCCTG
  CCTGACCCGGGAGGTCCTGCAGGGAATGCGGCCCAAGCTGGTCGGGTGAGCAGGCCAAAA
  GCTGTGGGGTCAGATCCGGAAGCTTTCCTCCCGGCCATCGATGTGGCGGAGCCGCAAGTA
  GACGTCCGTGCCAATGCCCTGCAAGGACTGCAGCTGCAGAGACCCACCGAGGTACTCCGC
  GTAGGCCCGTGACGTGGGCAACCCGAAGCCAAAGCCGTGCATGGGTCCTGACTGGGCGCC
  ACTATGCATGTCCAGATGGCCAAAGAGGGGGCTGATCCGGGGGTCCTGTGTGCTGGCCTC
  AGCAGTAGTGAAGTGGTAGTCCATGACCCGGTCCAGATCTTTGTGAGCGATTCCTCCACC
  ACGGTCTGAGATCCTGATGATCAGATCGACATCATTGTTGGCGATGGTGATGACCACATC
  TGGGACATTGTAAGGAGTGTCTAGGTGACTCTCCATTGTGGCTCTCATGGCATTCTTGAG
  CAGCTCCGGCAGGATGTAGTCCAGTGGCATAAGGATGAATGGGAACCGGGCAGCCACATG
  GCCATTGATGCCGACACGGGGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005881
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005881.1, NP_005872.1
• RefSeq Size: 1890 bp
• RefSeq ORF: 1239 bp
• Locus ID: 10295
• UniProt ID: O14874
• Cytogenetics: 16p11.2
• Domains: HATPase_c
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein encoded by this gene is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
  Transcript Variant: This variant (1) encodes the longest isoform (a).