Arginase 1 (ARG1) (NM_000045) Human Untagged Clone

CAT#: SC114331

ARG1 (untagged)-Human arginase, liver (ARG1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Arginase 1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114331 sequence for NM_000045 edited (data generated by NextGen Sequencing)
  ATGAGCGCCAAGTCCAGAACCATAGGGATTATTGGAGCTCCTTTCTCAAAGGGACAGCCA
  CGAGGAGGGGTGGAAGAAGGCCCTACAGTATTGAGAAAGGCTGGTCTGCTTGAGAAACTT
  AAAGAACAAGAGTGTGATGTGAAGGATTATGGGGACCTGCCCTTTGCTGACATCCCTAAT
  GACAGTCCCTTTCAAATTGTGAAGAATCCAAGGTCTGTGGGAAAAGCAAGCGAGCAGCTG
  GCTGGCAAGGTGGCAGAAGTCAAGAAGAACGGAAGAATCAGCCTGGTGCTGGGCGGAGAC
  CACAGTTTGGCAATTGGAAGCATCTCTGGCCATGCCAGGGTCCACCCTGATCTTGGAGTC
  ATCTGGGTGGATGCTCACACTGATATCAACACTCCACTGACAACCACAAGTGGAAACTTG
  CATGGACAACCTGTATCTTTCCTCCTGAAGGAACTAAAAGGAAAGATTCCCGATGTGCCA
  GGATTCTCCTGGGTGACTCCCTGTATATCTGCCAAGGATATTGTGTATATTGGCTTGAGA
  GACGTGGACCCTGGGGAACACTACATTTTGAAAACTCTAGGCATTAAATACTTTTCAATG
  ACTGAAGTGGACAGACTAGGAATTGGCAAGGTGATGGAAGAAACACTCAGCTATCTACTA
  GGAAGAAAGAAAAGGCCAATTCATCTAAGTTTTGATGTTGACGGACTGGACCCATCTTTC
  ACACCAGCTACTGGCACACCAGTCGTGGGAGGTCTGACATACAGAGAAGGTCTCTACATC
  ACAGAAGAAATCTACAAAACAGGGCTACTCTCAGGATTAGATATAATGGAAGTGAACCCA
  TCCCTGGGGAAGACACCAGAAGAAGTAACTCGAACAGTGAACACAGCAGTTGCAATAACC
  TTGGCTTGTTTCGGACTTGCTCGGGAGGGTAATCACAAGCCTATTGACTACCTTAACCCA
  CCTAAGTAA
  
  Clone variation with respect to NM_000045.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000045 unedited
  TTCACATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGCTCAAGTG
  CAGCAAAGAGAAGTGTCAGAGCATGAGCGCCAAGTCCAGAACCATAGGGATTATTGGAGC
  TCCTTTCTCAAAGGGACAGCCACGAGGAGGGGTGGAAGAAGGCCCTACAGTATTGAGAAA
  GGCTGGTCTGCTTGAGAAACTTAAAGAACAAGAGTGTGATGTGAAGGATTATGGGGACCT
  GCCCTTTGCTGACATCCCTAATGACAGTCCCTTTCAAATTGTGAAGAATCCAAGGTCTGT
  GGGAAAAGCAAGCGAGCAGCTGGCTGGCAAGGTGGCAGAAGTCAAGAAGAACGGAAGAAT
  CAGCCTGGTGCTGGGCGGAGACCACAGTTTGGCAATTGGAAGCATCTCTGGCCATGCCAG
  GGTCCACCCTGATCTTGGAGTCATCTGGGTGGATGCTCACACTGATATCAACACTCCACT
  GACAACCACAAGTGGAAACTTGCATGGACAACCTGTATCTTTCCTCCTGAAGGAACTAAA
  AGGAAAGATTCCCGATGTGCCAGGATTCTCCTGGGTGACTCCCTGTATATCTGCCAAGGA
  TATTGTGTATATTGGCTTGAGAGACGTGGACCCTGNGGAACACTACATTTTGAAAACTCT
  AGGCATTAAATACTTTTCAATGACTGAAGTGGACAGACTANGAATTGGCAAGGTGATGGA
  AGAAACACTCAGCTATCTACTAGGAAGAAAGAAAGGCCATTCATCTAGTTTTGATGTTGA
  CGGACTGGACCCATCTTTCACACCAGCTACTGGCACACCAGTCGTGGGAAGGTCTGACAT
  ACAGNAAANGGTCTCTACATCACAGAAGAAATCTACAAACAGGGCTACTCTNCAGATTAG
  ATTAATGGAAGTGAACCATNCCTGGGNNAGACACAGAGAAGTACTCGAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000045 unedited
  NNCCCGGGANNNNNCNNAAANAAAAAAAAAACTGACTTGNACCGCGCCGCATACTANGAT
  CGGTTTTTTTTTTTTTTTTTTTCTTGTTATAAAAATTTATTATAAAAAACACATTTTTTG
  AATGACATGGACACATAGTACCTTTCCACATGTGGTAGCTTTGTCTCCTGATTCCAAGAG
  TCCCACTCTATGTATGGGGGCTTATGTAAGTGTGCTTATTTTTAATGCCAGCAAATTTTT
  AATTGGAAATCAATGTACACTTCTCTTTCTAAGGATAAGTCTTTTGCCAAGTTAGAAAAT
  ATAAGCTTTTAAATTTCAAAAAAGTTAGAATTTCCACATCTTGAATTTTACACCAAGAGG
  GAATTTGTAGAGTTTATACTAATTGGAAAAACATTTTTCTGAAAGAACAAGTCTTTAGAA
  GGATAACTCTATGCTTAAGAAAATGATTAGCTTTCTAATTATGCCATTAACTATGAGATT
  TATATCGGATGTTTCCACATTTACTTAGGTGGGTTAAGGTAGTCAATAGGCTTGTGATTA
  CCCTCCCGAGCAAGTCCCGAAACAAGCCAAGGTTATTGCAACTGCTGTGTTCACTGTTCG
  AGTTACTTCTTCTGGTGTCTTCCCCAGGAATGGGTTCACTTCCATTATATCTAATCCTGA
  GAGTAGCCCTGTTTTGTAGATTTCTTCTGTGATGAAGAGACCCTCCTCTGTATGTCAGAC
  CTCCACGACTGGTGTGCCAGAAGCTGGTGTGAAAGATGGTCCACGCCCGCCACATCAAAA
  CTTAATGAATTGCCCTTTTCTTTTTTCCTAAGAGAAGCTGAATGTTTCTTCCATCACCTT
  GCCAATTCTAAACTGGCCCCTTAGCAATTGAAAAGATTTAATGCCCTAAGTTTCAAAAAT
  GTAGTTTCCCAGGGTCCCCTTTTTTAAGCCAATTAACCAATTTTCTTGCCCAAATTCCGG
  GAGCACCCCAGGAAAACCGGGCAATCGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000045
• Insert Size: 1570 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000045.2, NP_000036.2
• RefSeq Size: 1447 bp
• RefSeq ORF: 969 bp
• Locus ID: 383
• UniProt ID: P05089
• Cytogenetics: 6q23.2
• Domains: arginase
• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• Gene Summary: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.