DARS2 (NM_018122) Human Untagged Clone

CAT#: SC113685

DARS2 (untagged)-Human aspartyl-tRNA synthetase 2, mitochondrial (DARS2), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DARS2
• Synonyms: ASPRS; LBSL; MT-ASPRS; mtAspRS
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018122, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTACTTCCCTTCTTGGTTAAGTCAGCTGTACAGGGGTTTATCCAGACCCATCAGAAGGACCACCCAAC
  CGATCTGGGGTTCTCTCTACAGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTT
  TGTTGTCCGGACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA
  TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTTCAAGTTATCA
  TTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCCCCTGTGGAATCTGTGGTGCA
  AGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAGAATCCAAAAATGCCAACAGGTGAGATTGAA
  ATCAAAGTTAAAACAGCTGAGCTTCTGAATGCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGA
  AGAAAACAGAGGCTCTTCGGTTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCT
  GCGACTGAGGTCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA
  GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCCAGGGAACCTG
  GAAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTGATGGTTGGCGGTTTAGACAG
  ATATTTTCAGGTTGCCCGATGTTATCGAGATGAAGGTTCAAGACCAGACAGACAGCCTGAGTTTACTCAG
  ATTGACATAGAGATGTCATTTGTAGACCAGACTGGGATCCAGAGTTTAATTGAGGGTTTGCTCCAGTATT
  CCTGGCCCAATGACAAAGATCCTGTGGTTGTTCCTTTTCCTACTATGACTTTTGCTGAGGTGCTGGCCAC
  CTATGGAACTGATAAACCTGACACTCGCTTTGGAATGAAGATTATAGATATCAGTGATGTGTTTAGAAAC
  ACAGAGATTGGATTTCTTCAAGATGCACTTAGTAAGCCCCATGGAACTGTGAAAGCCATATGTATCCCTG
  AAGGAGCAAAATACTTAAAAAGGAAAGACATTGAATCCATTAGAAACTTTGCAGCTGACCATTTTAATCA
  GGAAATCTTACCTGTATTCCTTAACGCCAATAGAAACTGGAATTCTCCAGTTGCTAATTTCATAATGGAG
  TCACAAAGACTGGAATTAATCAGACTAATGGAGACCCAAGAGGAAGATGTGGTCCTACTAACTGCTGGAG
  AGCACAATAAAGCATGCTCTTTGTTAGGAAAATTACGACTGGAATGTGCTGACCTTCTAGAAACAAGAGG
  AGTGGTGCTCCGTGACCCCACTCTGTTCTCTTTCCTTTGGGTGGTAGATTTCCCACTCTTCCTGCCCAAG
  GAGGAAAATCCCAGAGAGCTGGAATCGGCCCACCACCCATTTACTGCTCCCCACCCCAGTGACATACATC
  TCCTGTACACTGAGCCCAAAAAGGCCCGTAGCCAACACTATGACTTGGTTTTAAATGGCAATGAAATAGG
  AGGTGGTTCAATTCGAATTCACAATGCAGAGCTGCAGCGTTATATCCTGGCAACCTTACTAAAGGAGGAT
  GTGAAAATGCTCTCCCATCTGCTCCAGGCTTTAGATTATGGGGCACCCCCTCATGGAGGAATTGCCTTAG
  GGTTAGACAGACTGATATGCCTTGTCACTGGATCTCCAAGCATCAGAGATGTCATAGCCTTCCCAAAGTC
  CTTCCGGGGACATGACCTCATGAGCAATACCCCAGATTCTGTCCCTCCTGAGGAACTGAAGCCCTATCAT
  ATCCGAGTCTCCAAGCCAACAGACTCCAAAGCAGAAAGAGCTCATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018122 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTCTCTCTAC
  AGAAGTCTGTTGCAGAGTTCACAGAGGAGAATTCCAGAATTCAGTAGCTTTGTTGTCCGG
  ACCAACACATGTGGAGAGTTGCGTTCGTCTCACTTAGGCCAAGAAGTCACCTTGTGTGGA
  TGGATTCAGTACCGAAGGCAAAACACATTCTTGGTCCTAAGAGATTTCGATGGGCTTGTT
  CAAGTTATCATTCCCCAGGATGAGTCGGCAGCCTCTGTGAAGAAGATTTTATGTGAAGCC
  CCTGTGGAATCTGTGGTGCAAGTGTCTGGTACAGTCATTTCCCGTCCTGCAGGACAAGAG
  AATCCAAAAATGCCAACAGGTGAGATTGAAATCAAAGTTAAAACAGCTGAGCTTCTGAAT
  GCCTGCAAGAAGCTGCCCTTTGAAATTAAGAACTTCGTGAAGAAAACAGAGGCTCTTCGG
  TTGCAGTATCGCTACTTAGACTTGCGTAGTTTCCAAATGCAGTATAACCTGCGACTGAGG
  TCCCAGATGGTCATGAAAATGCGGGAATATCTCTGTAATCTGCATGGGTTTGTGGATATA
  GAAACCCCCACATTGTTTAAGAGGACCCCAGGGGGTGCCAAAGAGTTTTTAGTACCATCC
  AGGGAACCTGGNAAGTTTTATTCTCTCCCTCAGAGTCCTCAACAGTTTAAGCAACTTCTG
  ATGGTTGGCGGGTTAGACAGATATTTTCAAGTTGCCCGATGTTATCGAGATGAAGTTTCA
  AGACCAGACAGACAGCCCTNAGTTTACTCAGATTGACATAGAGATGTCATTTGTAGACCA
  GACTGGGATCCAGAGTTTATATGAGGGTTTGCTCCAGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018122 unedited
  GGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTATAATTATTAGTTTATTAAAAC
  TTTTATGTGTATGATTAGAACTTAAAATCACAATGAGTTTTGGTGTTCACTTTTTCGCAC
  ATTTGAGGTTAATCTAAGGTTGATTAGAATTTTCAATTACTTATAATTGAGAGAAAATAG
  TGTTCAGTGATTAATAATATCTTTGTTGTCTTTTTTTTTTTTGAGATGGAGTCTTGCTCT
  GTCACCCAGGCTGGAGTGTAATGGCACCATCTTGGCTCACTGCAACCTCCATCTCCCAGG
  TTCAAGCAAACCTCATGCCTCAGCCTCCCGAGTGGCTGCGATTACAGGCATGCGCCACCA
  TGCCTGGCTAATTTTTGTGTTTTTAGTAGAGACGGTTTCACTATGTTGGCCAGGCTGGTG
  TTGAACTCCTGACCTCAAGTGATCCACCCACCTCAATCTCCCAAAGTGCTGGGATTACAG
  GTGTGAGCCACCACTCCCGGCCAATAATATCTTTAAAACTAAAAAAAAAACAGAAAATTG
  ATAAGTAAAATGACTTTTACTATTGATATCAGTTNTGTTTAATCTGAAACTATAAAATAG
  AGATATATCTACCTATTTATGGATGGATAATACCATCATCTCATATTTGGGGAATGCTTT
  ATAGTTTTTCAAAGTACTTTTATTCACTTTATCTTCCATATATGGGATCCTCTCTGATTC
  CAGCAATAACAGCCCGGTGAGGTAGCCAGGGCAAGTATGTTTTTTACACTTTAACAGGAA
  GGGAAGCTAAGCGAGGTTTATGTACTTACTCAGCCTGAAACACTGAAGAAAAATTGTGAC
  TCTCATTTCAGGGAGGTTTCTGCTTATTAAAAATTTAGCCCCTCCTCCTTATATTGGGGG
  GGTGGAAGGCCTTTTAACCTAATTTAATGATCCTCTGCGAGGGGAGAAACATGGTCCTTC
  CTCCTATTTACGGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018122
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018122.1, NP_060592.1
• RefSeq Size: 3348 bp
• RefSeq ORF: 3348 bp
• Locus ID: 55157
• UniProt ID: Q6PI48
• Cytogenetics: 1q25.1
• Domains: GAD, tRNA-synt_2
• Protein Pathways: Aminoacyl-tRNA biosynthesis
• Gene Summary: The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL). [provided by RefSeq, Nov 2009]