TMEM35 (TMEM35A) (NM_021637) Human Untagged Clone

CAT#: SC112910

TMEM35 (untagged)-Human transmembrane protein 35 (TMEM35)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TMEM35
• Synonyms: NACHO; TMEM35; TUF-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112910 sequence for NM_021637 edited (data generated by NextGen Sequencing)
  ATGGCATCCCCCAGAACCGTAACTATTGTGGCCCTCTCAGTGGCCCTGGGACTCTTCTTT
  GTTTTCATGGGGACTATCAAGCTGACCCCCAGGCTCAGCAAGGATGCCTACAGTGAGATG
  AAACGTGCTTACAAGAGCTATGTTCGAGCCCTCCCTCTGCTGAAGAAAATGGGGATCAAT
  TCCATTCTCCTCCGAAAAAGCATTGGTGCCCTTGAAGTGGCCTGTGGCATCGTCATGACC
  CTTGTGCCTGGGCGTCCCAAAGATGTGGCCAACTTCTTCCTACTGTTGCTGGTGTTGGCT
  GTGCTCTTCTTCCACCAGCTGGTCGGTGATCCTCTCAAACGCTACGCCCATGCTCTGGTG
  TTTGGAATCCTGCTCACTTGCCGCCTGCTGATTGCTCGCAAGCCCGAAGACCGGTCTTCT
  GAGAAGAAGCCTTTGCCAGGGAATGCTGAGGAGCAACCCTCCTTATATGAGAAGGCCCCT
  CAGGGCAAAGTGAAGGTGTCATAG
  
  Clone variation with respect to NM_021637.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021637 unedited
  GTAAAGGACGTACCGTTGTATAACGCCTCCTAATAGGGCGGCCCGCGACTCTTCGGCAAC
  GAGTCCTCGTGCCGAATTCGGCACGAGGCCTTTGTCATTCTAGCTGCCTGCTGCCTCCGC
  AGCGTCCCCCCAGCTCTCCCTGTGCTAACTGCCTGCACCTTGGACAGAGCGGGTGCGCAA
  ATCAGAAGGATTAGTTGGGACCTGCCTTGGCGACCCCATGGCATCCCCCAGAACCGTAAC
  TATTGTGGCCCTCTCAGTGGCCCTGGGACTCTTCTTTGTTTTCATGGGGACTATCAAGCT
  GACCCCCAGGCTCAGCAAGGATGCCTACAGTGAGATGAAACGTGCTTACAAGAGCTATGT
  TCGAGCCCTCCCTCTGCTGAAGAAAATGGGGATCAATTCCATTCTCCTCCGAAAAAGCAT
  TGGTGCCCTTGAAGTGGCCTGTGGCATCGTCATGACCCTTGTGCCTGGGCGTCCCAAAGA
  TGTGGCCAACTTCTTCCTACTGTTGCTGGTGTTGGCTGTGCTCTTCTTCCACCAGCTGGT
  CGGTGATCCTCTCAAACGCTACGCCCATGCTCTGGTGTTTGGAATCCTGCTCACTTGCCG
  CCTGCTGATTGCTCGCAAGCCCGAAGACCGGTCTTCTGAGAAGAAGCCTTTGCCAGGGAA
  TGCTGAGGAGCAACCCTCCTTATATGAGAAGGCCCCTCAGGGCAAAGTGAAGGTGTCATA
  GAAAAGTGGAAGTGCAAAGAGTGGACCTTCCAGGCAGTTGCGTCCATGACACCAGGAAGA
  TGTCAGTGTGTGTTTTTCATTTGATTTATTTATCTTGGGGAAAGTGAAAAATGTAATCTG
  CAAGTTAATGACCCTATTGGCTTGTGTACATCTATATGCTAAAATGACTTCCCCACATTG
  ACATTTGTGCGCCACCTTTTATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021637 unedited
  TATGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCA
  AATTTCAAAAAGTTTTATTTTGAAAGAATGAGAGAAATAAAACAGAGAGGTTTCAATTAC
  CAAAAACAATTACACTGAAAAAAACACAATAATAAGTACTTTTCCCACACAACCCCCCCC
  ATTTCCCCATCCCTGGCACAATAATATTAAAACCACCAAAGCACACCTAACAAGGAAAAA
  CAACAGTACGTAATGAAAAAAGCAAATGTCCATACTGCTCAGTCCAACTAACCCTTATGA
  AATGTCCTTCCCCCAGCTAAACCCTACCCACTGGAATGATAAAAAAATGTAAAGACAACC
  CTAGGGGAAACTTGGAACTCTGCTTATACTAGCAAAGCTCAGTGAAAAATCAGTAAAAGT
  AGTGAATCTGTTTGGCAGTGAAACACTGGATATAGCTTCTTTTTCAAATTTTGGATGATT
  GCAAAAAACAGGTAAAGTTTGAGGCTCACAGACTTCTAACAGGACTGATCCCTGTTCCCT
  CAACCGTAACAGTGGAGCAGCTGCCAAATCCTGGTTGGCTGCTGAAAATAGTGGAAGTGA
  GGCAACTTTGGGCTTAAGACAATCTGAAAAAGATGTCTGGAAACAAGTTTACTGCTTCAG
  TTTCAAACTGTGAGTAAAGGGTTTTAATAGGTGGGGCTTGGACAAAGGCAGGTTGAAACT
  TCCATATTATCACTTGAAGAATTAAACTATCAATTTGACTGCATGCATCCCCTTGTTTTA
  AATAAATTCAATATGGGACTATTTAAAAATGTAAACNAACTGTTGGNACTAGAGCCTAAT
  TAAATTCATATANTAACTCANTGTCCGAAAATGAATGTTTTTTTTTTTTCTCCTAAGTAA
  TAAAGTTTAGNTTGTAGTATCTGGTGGAAAGATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021637
• Insert Size: 504 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021637.1, NP_067650.1
• RefSeq Size: 2039 bp
• RefSeq ORF: 504 bp
• Locus ID: 59353
• UniProt ID: Q53FP2
• Cytogenetics: Xq22.1
• Protein Families: Transmembrane
• Gene Summary: A soluble peptide released by shedding may interact with NGFR and modulate neurite outgrowth.[UniProtKB/Swiss-Prot Function]